| 鱼腥草多糖对MA104和MARC145细胞活力影响的比较研究 |
| |
| 引用本文： | 朱琪,李秀丽,任卫科,江 珊,张 莉,王建国,鄢明华. 鱼腥草多糖对MA104和MARC145细胞活力影响的比较研究[J]. 中国兽药杂志, 2022, 56(3): 60-65 |
| |
| 作者姓名： | 朱琪 李秀丽 任卫科 江 珊 张 莉 王建国 鄢明华 |
| |
| 作者单位： | 天津市农业科学院 畜牧兽医研究所,,,,,,天津市农业科学院 畜牧兽医研究所 |
| |
| 摘 要： | 研究鱼腥草多糖(houttuynia cordata polysaccharide,HCP)对MA104和MARC145细胞活力的影响,筛选梯度浓度的鱼腥草多糖对MA104和MARC145细胞促生长最佳浓度剂量范围和毒性剂量.通过水提醇沉法提取HCP,得糖率为8.18％;分别采用MTT法和细胞平板计数法绘制MA104和...
|
| 关 键 词： | 鱼腥草多糖 MA104细胞 MARC145细胞 细胞活力 MTT法 |
| 收稿时间： | 2021-08-12 |
| 修稿时间： | 2021-12-27 |
Effects of Houttuynia Cordata Polysaccharides on Viability of MA104 and MARC145 Cells |
| |
| Affiliation: | Tianjin Institute of Animal Husbandry and Veterinary,,,,,, |
| |
| Abstract: | (Objective)The effects of Houttuynia cordata polysaccharide(HCP)on viability of MA104 and MARC145 cells were studied in this experiment. (Method)The sugar yield of HCP was 8.18% by water extraction and alcohol precipitation. The growth curves of MA104 and MARC145 cells were plotted by MTT method and cell counting method, respectively. MTT assay was used to detect the effects of gradient concentration of HCP on viability of MA104 and MARC145 cells. (Result)The results showed that viability of MA104 and MARC145 cells were inhibited by 50 mg/mL HCP, these were promoted by 0.05~5 mg/mL HCP, and these were no significant effect by 0~0.005 mg/mL HCP. (Conclusion)Conclusion: Below 5 mg/mL HCP had no toxic effect on MA104 and MARC145 cells. |
| |
| Keywords: | houttuynia cordata polysaccharide MA104 cells MARC145 cells cell viability MTT assay |
|
| 点击此处可从《中国兽药杂志》浏览原始摘要信息 |
|
点击此处可从《中国兽药杂志》下载全文 |
|
