不同油气储藏区微生物代谢活性及多样性响应

【目的】 研究土壤微生物群落代谢活性及多样性对地下油气储藏情况的响应。【方法】 采用Biolog-ECO微平板技术,研究焉耆境内不同油气储藏地区,地下80~100 cm土壤样品微生物的群落代谢活性及多样性。【结果】 微生物代谢活性总体呈现利于藏油气区域微生物活性>不利于或难于评价藏油气区域>无藏油气区域的规律;在利于藏油气和不利于或难评价藏油气区域样品中,氨基酸和多聚物利用微生物在AWCD的贡献上均高于对照区域,而羧酸、胺类均低于对照区域。主成分分析表明,三个不同藏油气区域微生物群落分布特征有着明显差异,PCA 1、PCA 2中权重最大的基质为D-甘露醇和D-木糖,分别为载荷值0.97和0.87。【结论】 新疆焉耆三个区域,地下土壤微生物群落明显受油气分布影响。Biolog -ECO微平板技术可能作为油气储藏初步勘测的一种手段。

Microbial Metabolic Activity and Diversity Response in Different Oil and Gas Storage Areas

【Objective】 To study the response of soil microbial community metabolic activity and diversity to underground oil and gas storage.【Method】 The metabolic activity and diversity of microbial community in 80-100 cm soil samples from different oil and gas storage areas in Yanqi were studied by using Biolog-ECO microplate technique.【Result】 In the three areas of Yanqi, Xinjiang that the study analyzed, soil microbial communities were obviously affected by oil and gas distribution. The general rules of microbial metabolic activity were as follows: the microbial activity of favorable reservoir oil and gas region > unfavorable or difficult to evaluate reservoir oil and gas area > no reservoir oil and gas area; The contribution of amino acid and polymer utilization microbes to AWCD in the samples that were favorable to reservoir oil and gas or difficult to evaluate was higher than that in the control area, while carboxyl and amines were lower than those in the control area. Results of principal component analysis showed that the distribution characteristics of microbial communities in three different reservoirs were obviously different, and the most powerful matrixes was the D-mannitol and D-xylose in PCA 1 and PCA 2, whose respective load values were 0.97 and 0.87.【Conclusion】 Biolog-ECO microplate technology can be used as a means for preliminary exploration of oil and gas reservoirs.