Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon |
| |
Authors: | P H Bayemi V M Nsongka B M A O Perera D Cavestany E C Webb |
| |
Institution: | (1) Institute of Agricultural Research for Development, Bambui B.P. 51, Bamenda, Cameroon;(2) University of Peradeniya, Peradeniya, Sri Lanka;(3) Instituto Nacional de Investigación Agropecuaria, INIA La Estanzuela, C. Correos, Uruguay;(4) Department of Animal and Wildlife Sciences, University of Pretoria, Pretoria, South Africa |
| |
Abstract: | A study aimed at validating a human progesterone enzyme immunoassay kit was carried out on cattle at Bambui, Cameroon. Progesterone
ELISA Kits (EH-511) were obtained from Clinpro International. Forty-one cows were selected, of which 19 were pregnant and
22 within 14 days post partum. Blood samples were analysed and progesterone levels were deduced from a curve obtained from standard absorbance values (A
450). Results show that 95.5% of postpartum cows had progesterone levels below 1 ng/ml, with the highest level being 0.75 ng/ml.
The mean level was 0.5 ± 0.26 ng/ml. The cows in the ‘pregnant group’ had progesterone levels ranging from 3.5 to 17.5 ng/ml.
This kit can be used for measuring progesterone levels in cattle. Levels of 1 ng/ml for two consecutive samples or one sample
at or above 3 ng/ml are an indication of the presence of corpus luteum, while cows below 1 ng/ml will be in anoestrus. |
| |
Keywords: | Cameroon Cattle ELISA Immunoassay Progesterone Validation |
本文献已被 PubMed SpringerLink 等数据库收录! |
|