Serological relationships of geminivirus isolates from Gramineae in Australia |
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| Authors: | M S PINNER P G MARKHAM E P RYBICKI R S GREBER |
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| Institution: | John Innes Institute, John Innes Centre for Plant Science Research, Colney Lane, Norwich NR4 7UH, UK;Department of Microbiology, University of Cape Town, PB Rondebosch, 7700, South Africa;Queensland Department of Primary Industries, Maroochy Horticultural Research Station, Nambour, Queensland, Australia |
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| Abstract: | Three distinct viruses, named chloris striate mosaic virus (CSMV), paspalum striate mosaic virus (PaSMV) and digitaria didactyla striate mosaic virus (DDSMV), have been identified among the five Gramineae-infecting geminiviruses from Australia using polyclonal antisera. An isolate from Microlaena was confirmed as a strain of CSMV, and an isolate from Bromus catharticus was identified as PaSMV-BC. Enzyme-linked immunosorbent assay (ELISA) detected relationships between all but one of the viruses tested, the exception being miscanthus streak virus (MiSV) from Japan. The Australian viruses proved to be distantly related to similar viruses from Africa, digitaria streak virus (DSV) from Vanuatu, and wheat dwarf virus (WDV) from Europe. Three distinct groups of viruses from Africa, Australia and Europe were distinguished by phylogenetic analysis. |
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