| 基因瞬时表达技术在园艺植物上的应用研究进展 |
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| 引用本文： | 崔霞,张率斌.基因瞬时表达技术在园艺植物上的应用研究进展[J].园艺学报,2017,44(9):1787-1795. |
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| 作者姓名： | 崔霞 张率斌 |
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| 作者单位： | （沈阳农业大学园艺学院，设施园艺省部共建教育部重点实验室，环渤海湾地区设施蔬菜优质高效生产协同创新中心，沈阳 110866） |
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| 基金项目： | 国家自然科学基金面上项目(31572140) |
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| 摘 要： | 基因瞬时表达技术是近年来发展形成的一种快速高效的基因功能分析方法，应用广泛。本文综述了基因瞬时表达技术的原理、影响因素及其在园艺植物上的应用，指出该技术在当前应用中存在的不足并提出建议及展望。
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| 关 键 词： | 园艺植物 瞬时表达 农杆菌介导法 病毒诱导基因沉默 |
The Utilization and Prospect of Genome Editing in Horticultural Crops |
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| CUI Xia,ZHANG Shuaibin.The Utilization and Prospect of Genome Editing in Horticultural Crops[J].Acta Horticulturae Sinica,2017,44(9):1787-1795. |
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| Authors: | CUI Xia ZHANG Shuaibin |
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| Institution: | Transient expression technology is a rapid and efficient technology of large-scale function analysis，with extensive application. The paper reviewed the principles of transient expression system，influencing factors，and applications in horticultural plants. Meanwhile it also proposed some deficiencies which still existed in this system，and put forward some recommendations and future applications in horticultural plants. |
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| Abstract: | Genome editing is a new technology based on programmable nucleases to make precise changes in the genomes of cells.These nucleases create site-specific double-strand breaks (DSBs) at desired locations in the genome.The induced double-strand breaks are repaired through nonhomologous end-joining (NHEJ) or homologous recombination (HR),resulting in targeted mutations including deletion,insertion and exchange of specific DNA fragment.Here,we explained the principle of ZNF,TALEN and CRISPR/Cas9 and mainly summarized their usage in plants,especially in horticultural plants.As a precise and efficient method of genetic engineering,genome editing was developed rapidly in recent years,which will be utilized widely to improve agronomic traits of plant in the future. |
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| Keywords: | genome editing horticultural crops utilization prospect |
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