茌梨套PE袋和无纺布袋对果点及贮藏失水的影响机理

观察茌梨套绿色聚乙烯(PE)薄膜袋和无纺布袋栽培的果实果点及果皮亚显微结构,并测定相关生理指标、基因表达量等,探究不同套袋处理对茌梨果点及果实贮藏失水的影响机理。结果表明:套绿色PE薄膜袋的果实,果面粗糙,果点大;而套无纺布袋的果实果面光洁,果点小。果实发育后期,套绿色PE薄膜袋的果实果皮中PbPAL2基因的表达量高于套无纺布袋果实,其贮藏期间质量损失率显著高于对照,果皮较薄,表面蜡质少且龟裂翘起;而套无纺布袋果实质量损失率较低,果皮厚且表面蜡质多,蜡质晶体形态多样。采前套袋通过调控PbPAL2的表达进而影响果点的木栓化,并改变了果皮角蜡层结构影响其贮藏失水。采前套无纺布袋有利于提高茌梨果实的外观品质,延缓其贮藏失水。

The Influence Mechanism of PE and Non-woven Fabric Bagging Treatments on Fruit Spot and Water Loss During Storage of'Chili'Pear

To explore the influence mechanism of different bagging treatments on the'Chili'pear (Pyrus bretschneideri Rehd.'Chili') fruit spot and water loss during storage,the appearance and microstructure of polyethylene (PE)-bagged and non-woven fabric-bagged fruit were observed,and the physiological indexes as well as gene expression level were determined.The results showed that the PE-bagged fruit surface was rough and the fruit spot was big,while the non-woven fabric-bagged fruit surface was smooth and the fruit spot was small,and the gene expression level of PbPAL2 in the pericarp of PE-bagged fruit was higher than non-woven fabric-bagged fruit.The weight loss rate of PE-bagged fruit was higher than the control during storage,the pericarp was thinner,with less wax substance,and the surface of pericarp was cracked and cocked.On the contrary,the weight loss rate of non-woven fabric-bagged fruit was lower than PE-bagged fruit during storage,the pericarp was thick,with more wax substance on the surface of pericarp,and the wax crystal was diverse.Preharvest bagging influenced the suberification of fruit spot by regulating the gene expression pattem of PbPAL2,and influenced the water loss during storage by changing the cuticle layer and wax layer structure of pericarp.Preharvest non-woven fabric-bagging treatment was beneficial to improve the fruit appearance quality and delay the water loss of'Chili'pear during storage.