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| 苹果抗炭疽菌叶枯病基因相关的4个分子标记的准确性验证 | |
| 引用本文： | 刘春晓,兰进好,侯鸿敏,张玉刚,戴洪义,刘源霞.苹果抗炭疽菌叶枯病基因相关的4个分子标记的准确性验证[J].园艺学报,2017,44(7):1355-1362. |
| 作者姓名： | 刘春晓 兰进好 侯鸿敏 张玉刚 戴洪义 刘源霞 |
| 作者单位： | 1 青岛农业大学园艺学院，青岛市园艺植物遗传改良与育种重点实验室，山东青岛 266109；2 青岛农业大学农学 与植物保护学院，山东青岛 266109 |
| 基金项目： | 国家自然科学基金项目(31372032)，国家现代农业产业技术体系建设专项资金项目(CARS-28-01-07)，山东省良种产业化工程项目(620902)，“泰山学者”建设工程和青岛市民生科技计划项目(15-9-2-99-nsh;14-2-3-38-nsh)，‘十二五’国家科技支撑计划项目(2013BAD02B01)，青岛农业大学高层次人才科研基金项目(6631117007)，山东省优秀中青年科学家科研奖励基金项目(BS2014SW027) |
| 摘 要： | 利用50个苹果栽培品种(系)对与抗炭疽菌叶枯病基因(R_(gls))位点紧密连锁的4个分子标记S0405127(SSR)、S0304673(SSR)、SNP4236和In Del4254的准确性进行验证。室内离体接种表型鉴定及分子标记基因型鉴定的结果表明,4个分子标记验证苹果炭疽菌叶枯病抗性的准确率分别为90%、94%、96%和92%。 |
| 关 键 词： | 苹果 炭疽菌叶枯病 分子标记 可靠性验证 |
Accuracy Test of Four Molecular Markers of Glomerella Leaf Spot Resistant Gene in Apple Cultivars |
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| LIU Chunxiao,LAN Jinhao,HOU Hongmin,ZHANG Yugang,DAI Hongyi,LIU Yuanxia.Accuracy Test of Four Molecular Markers of Glomerella Leaf Spot Resistant Gene in Apple Cultivars[J].Acta Horticulturae Sinica,2017,44(7):1355-1362. | |
| Authors: | LIU Chunxiao LAN Jinhao HOU Hongmin ZHANG Yugang DAI Hongyi LIU Yuanxia |
| Institution: | 1Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants，College of Horticulture， Qingdao Agricultural University，Qingdao，Shandong 266109，China； 2 College of Agronomy and Crop Protection，Qingdao Agricultural University，Qingdao，Shandong 266109，China |
| Abstract: | In the present study,the accuracy of the four molecular markers tightly linked with Rgls locus,S0405127 (SSR),S0304673 (SSR),SNP4236,and InDel4254 were tested based on the resistant phenotypic and genotypic identification of 50 apple cultivars.The results indicated that the accuracy of the four molecular markers were 90％,94％,96％,92％,respectively.The study could provide breeders an effective method to speed up breeding program by using these molecular markers for the pre-selection of the hybrid seedlings resistant to the Glomerella leaf spot in apple. |
| Keywords: | apple Glomerella leaf spot molecular marker accuracy test |
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