| 连翘长花柱与短花柱植株花粉形态特征和花中化学成分比较 |
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| 引用本文： | 房信胜,周红英,宁安琪,苏洁,王建华. 连翘长花柱与短花柱植株花粉形态特征和花中化学成分比较[J]. 园艺学报, 2017, 44(2): 373-380. DOI: 10.16420/j.issn.0513-353x.2016-0482 |
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| 作者姓名： | 房信胜 周红英 宁安琪 苏洁 王建华 |
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| 作者单位： | 山东农业大学农学院，作物生物学国家重点实验室，山东省作物生物学重点实验室，山东泰安 271018 |
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| 基金项目： | 中医药行业科研专项(201407002)，山东省重点研发计划项目(2016GSF202017)，山东省现代农业产业技术体系项目(SDAIT-20-04) |
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| 摘 要： | 以连翘(Forsythia suspense)长花柱和短花柱植株的花为试验材料,采用扫描电镜对花粉形态进行观察,采用高效液相色谱法测定花中6种化学成分的含量,采用福林酚比色法测定花中总多酚含量。结果表明,连翘花粉粒形状主要呈长球形、近球形和纺锤形。极面观多近圆形或三裂圆形,三条萌发沟延伸达两极,表面网状纹饰。生长环境对花粉粒大小的影响不显著。短花柱型植株的花粉粒极轴长和赤道轴长分别显著高于长花柱型的(P0.05)。环境对长、短花柱花中化学成分均有较大影响;长花柱型花中松脂醇–β–D–葡萄糖苷的含量显著高于短花柱型花(P0.05)。
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| 关 键 词： | 连翘 异型花柱 花粉形态 次生代谢产物 扫描电镜 高效液相色谱 |
Comparison of Pollen Morphology and Chemical Components of Flower Between Long Style Type and Short Style Type of Forsythia suspense |
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| FANG Xinsheng,ZHOU Hongying,NING Anqi,SU Jie,WANG Jianhua. Comparison of Pollen Morphology and Chemical Components of Flower Between Long Style Type and Short Style Type of Forsythia suspense[J]. Acta Horticulturae Sinica, 2017, 44(2): 373-380. DOI: 10.16420/j.issn.0513-353x.2016-0482 |
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| Authors: | FANG Xinsheng ZHOU Hongying NING Anqi SU Jie WANG Jianhua |
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| Affiliation: | College of Agronomy，Shandong Agricultural University，State Key Laboratory of Crop Biology，Shandong Key Laboratory of Crop Biology，Tai’an，Shandong 271018，China |
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| Abstract: | Pollen morphology and secondary metabolites of flower of long style type (LST) and short style type (SST) of Forsythia suspense were investigated in this study.Pollen grains were observed using scanning electron microscope;Six components in flower were determined using HPLC;Total phenol content in flower was determined using Folin-Ciocalteu reagent assay.The results showed that the pollen grains were prolate spheroid,subsphaeroidal,and spindle in shape,sub-rounded or 3-lobed-round type in polar view,3-colpate type,sexine ornamentation were reticulate.The influence of growing environment on the size of pollen grains was not significant.The length of polar axis and equator axis of pollen grains of SST was significantly higher (P ＜ 0.05) than that of LST.Content of chemical components in flower could be influenced by growing environment.The content of pinoresinol-β-D-glueoside in flower of LST was significantly higher (P ＜ 0.05) than that of SST. |
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| Keywords: | Forsythia suspense heterostyly pollen morphology secondary metabolites scanning electron microscopy HPLC |
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