| 桃FLA家族基因的生物信息学及表达分析 |
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| 引用本文： | 卞坤,邵佳蓉,徐利伟,沈子明,陈伟,杨震峰. 桃FLA家族基因的生物信息学及表达分析[J]. 园艺学报, 2017, 44(4): 653-663. DOI: 10.16420/j.issn.0513-353x.2016-0604 |
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| 作者姓名： | 卞坤 邵佳蓉 徐利伟 沈子明 陈伟 杨震峰 |
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| 作者单位： | 1. 上海海洋大学食品学院,上海201306;浙江万里学院生物与环境学院,浙江宁波315100;2. 浙江万里学院生物与环境学院,浙江宁波,315100 |
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| 基金项目： | 国家自然科学基金项目(31371866)，浙江省自然科学基金项目(LQ15C200004)，浙江省重中之重学科学生创新项目(1740002979) |
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| 摘 要： | 从桃基因组数据库筛选出了12个假定的成束状阿拉伯半乳糖蛋白(FLA)基因序列。对其蛋白结构进行分析,结果显示PpFLA7不具有特定的阿拉伯糖或半乳糖糖基化位点,不属于典型的FLA家族。PpFLA6的氨基酸组成和保守域的分布与其他成员相比差别较大。聚类分析结果表明PpFLAs家族可以分为CladeⅠ、CladeⅡ和CladeⅢ3类。利用荧光定量PCR(qPCR)对不同组织和成熟阶段果实中的FLA进行表达分析,发现各个成员在不同时期的组织中差异化表达,其中PpFLA4在果实成熟阶段显著上调表达,暗示其可能在桃果实的成熟阶段扮演重要角色。
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| 关 键 词： | 桃 果实 成束域 阿拉伯半乳糖蛋白 生物信息学 表达分析 |
Bioinformatic and Expression Analysis of Fasciclin-like Arabinogalactan Proteins (FLAs) Gene Family in Peach |
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| BIAN Kun,SHAO Jiarong,XU Liwei,SHEN Ziming,CHEN Wei,YANG Zhenfeng. Bioinformatic and Expression Analysis of Fasciclin-like Arabinogalactan Proteins (FLAs) Gene Family in Peach[J]. Acta Horticulturae Sinica, 2017, 44(4): 653-663. DOI: 10.16420/j.issn.0513-353x.2016-0604 |
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| Authors: | BIAN Kun SHAO Jiarong XU Liwei SHEN Ziming CHEN Wei YANG Zhenfeng |
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| Abstract: | Twelve Fasciclin-like arabinogalactan proteins (FLA) sequences were screened from the peach (Prunus persica) genome database.Protein structure analysis of the 12 FLAs revealed that PpFLA7 seems not the classical member of FLA family due to the absence of glycosylated sites.PpFLA6 showed a significant difference with other ones because of a distinctive composition and distribution of conserved domains.Phylogenetic analysis indicated that PpFLAs family could be classified into 3 groups:Clade Ⅰ,Clade Ⅱ,and Clade Ⅲ.Expression analysis by qPCR manifest that 12 PpFLAs were differentially expressed in various tissues,and the highest expression of PpFLA4 in firm-ripe peach fruit suggest that this member may play an important role in peach fruit during ripening. |
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| Keywords: | peach fruit fasciclin-like domain arabinogalactan protein bioinformatics expression analysis |
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