| 三裂叶葛毛状根的诱导及葛根素的产生 |
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| 引用本文： | 刘士平,沙爱华,薛艳红,李金泉,宋志红. 三裂叶葛毛状根的诱导及葛根素的产生[J]. 湖北农业科学, 2006, 45(2): 153-155 |
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| 作者姓名： | 刘士平 沙爱华 薛艳红 李金泉 宋志红 |
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| 作者单位： | 三峡大学化学与生命科学学院,湖北,宜昌,443002;中国农业科学院油料作物研究所,武汉,430062;湖北省农业科学院经济作物研究所,武汉,430064 |
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| 基金项目： | 三峡大学校科研和教改项目 |
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| 摘 要： | 利用发根农杆菌R1601感染药用植物三裂叶野葛叶片、茎段、芽、叶柄等外植体,诱导毛状根产生,通过PCR扩增和southern印迹试验,证实发根农杆菌Ri质粒的T-DNA片段已整合进植物的核基因组中,这些毛状根可以在无激素的培养基中快速生长.本试验探索了不同的外植体对诱导率的影响,对产生的毛状根活性物质的含量进行了分析,结果表明,葛转化中叶片是最合适的外植体,而不同的毛状根细胞株中葛根素的含量差异较大,平均含量达到4.21%.
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| 关 键 词： | 葛 毛状根 发根农杆菌 葛根素 |
| 文章编号： | 0439-8114(2006)02-0153-03 |
| 收稿时间： | 2006-01-10 |
| 修稿时间： | 2006-01-10 |
Induction of Hairy Roots in Pueraria phaseoloides and Its Puerarin Production |
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| LIU Shi-ping,SHA Ai-hua,XUE Yan-hong,LI Jin-quan,SONG Zhi-hong. Induction of Hairy Roots in Pueraria phaseoloides and Its Puerarin Production[J]. Hubei Agricultural Sciences, 2006, 45(2): 153-155 |
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| Authors: | LIU Shi-ping SHA Ai-hua XUE Yan-hong LI Jin-quan SONG Zhi-hong |
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| Affiliation: | 1. Chemistry and Life Science College, Three Gorges University, Yiehang 443002,Hubei,China; 2.Institute of Oil Crop, Chinese Academy of Agricultural Sciences, Wuhan 430070, China; 3.Institute of Economic Crops, Hubei Academy of Agricuhule Sciences,Wuhan 430064, China |
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| Abstract: | Hairy roots appeared after the co-culture of various Pueraria phaseoloides explants and Agrobacterium rhizogenes R1601.It was clearly confirmed that the T-DNA segment was integrated into the cells of hairy roots through PCR amplification and southern blotting.These transformants could grow rapidly on MS medium without hormone.This study also demonstrated the action of explants on the induction rate.The result showed that leaves were superior to other organs as stems,buds and petioles.The puerarin yield of different hairy strain varied largely,holding a 4.21% contents averagely. |
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| Keywords: | Pueraria phaseoloides hairy roots Agrobacterium rhizogenes puerarin |
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