雪花莲凝集素基因转化新疆甜瓜抗蚜虫的研究

采用农杆菌介导法对新疆甜瓜进行了遗传转化研究,对影响转化频率的不同因素,即:抗生素浓度、预培养有无及感染时菌液的不同处理、外植体与农杆菌的感染时间和共培养时间等进行了研究,通过农杆菌介导法将雪花莲凝集素基因导入四日龄甜瓜子叶外植体,获得了卡那霉素的抗性植株.移栽成活并生长良好.卡那霉素抗性鉴定及转化株总DNA的PCR分析表明,GNA基因已整合到甜瓜的基因组中.初步抗蚜虫实验证明,转基因甜瓜有一定的抗棉蚜幼虫效果.

A Study on Transferring Snowdrop Galanthus nivalis(GNA) Gene in Cucumis melo L.of Xinjiang

This study was designed to investigate the genetic transformation of Cucumis melo L.by Agrobacterium-mediated trans formation.Some factors affecting the Agrobacterium-mediated transformation frequency,i.e.concentrations of antibiotics,preculture of cotyledon explants,treatments of Agrobacterium and infection time,co-infection time were also discussed.GNA Gene was translated into melon by Agrobacterium-mediated transformation,The kanamycin-resistant plants have been regenerated.They survived after transplantation and grew well in pots.Transgenic plants were confirmed by the kanamycin-resistant experimen and PCR analysis.The preliminary results from bioassay demonstrated significant resistance of the transgenic plants to cotton aphide larvae.