| 猪CD86 mRNA定量检测方法的建立 |
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| 引用本文： | 周双海,杨汉春,郭鑫,陈艳红,查振林.猪CD86 mRNA定量检测方法的建立[J].北京农学院学报,2006,21(2):33-35. |
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| 作者姓名： | 周双海 杨汉春 郭鑫 陈艳红 查振林 |
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| 作者单位： | 中国农业大学,动物医学院,北京,100094;北京农学院,动物科学技术系,北京,102206;中国农业大学,动物医学院,北京,100094 |
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| 基金项目： | 教育部跨世纪优秀人才培养计划 |
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| 摘 要： | 用RT-PCR技术从猪肺泡巨噬细胞中扩增和克隆了猪CD86基因部分片段,经测序鉴定后,用反向PCR技术构建了CD86的缺失竞争cDNA分子。通过竞争PCR方法,建立了CD86标准竞争曲线,并得到其直线回归方程。本方法操作简单,特异性和敏感性高,可用于猪CD86 mRNA水平的定量检测。
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| 关 键 词： | 猪 CD86 竞争PCR |
| 文章编号： | 1002-3186(2006)02-0033-03 |
| 收稿时间： | 2005-10-10 |
| 修稿时间： | 2005-12-30 |
Development of Quantitative Detection of Porcine CD86 mRNA |
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| ZHOU Shuang-hai,YANG Han-chun,GUO Xin,CHEN Yan-hong,ZHA Zhen-lin.Development of Quantitative Detection of Porcine CD86 mRNA[J].Journal of Beijing Agricultural College,2006,21(2):33-35. |
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| Authors: | ZHOU Shuang-hai YANG Han-chun GUO Xin CHEN Yan-hong ZHA Zhen-lin |
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| Institution: | 1. College of Veterinary Medicine, China Agricultural University, Beijing 100094,China; 2. Department of Animal Science and Veterinary Medicine, Beijing Agricultural College, Beijing 102206, China |
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| Abstract: | Porcine CD86 was amplified by RT-PCR from porcine alveolar macrophages and cloned.Following sequencing,competitive depletion clones for the cDNA molecules was generated by reverse PCR.Then,the linear regression equation was obtained after construction of the standard competitive curves by quantitative competitive PCR assay.This study provided a simple,high specific and sensitive method for defecting porcine CD86 mRNA level. |
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| Keywords: | porcine CD86 competitive PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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