疏肝健脾解毒方对乳腺癌癌前病变肝郁证模型大鼠血清性激素水平的影响

目的 研究疏肝健脾解毒方对乳腺癌癌前病变肝郁证模型大鼠血清性激素水平的影响。方法 采用二甲基苯蒽灌胃及夹尾法制备乳腺癌癌前病变肝郁证大鼠模型，随机分为模型组、疏肝健脾解毒方组、三苯氧胺组、小金丸组，同时设空白对照组，分别干预4周后采用放射免疫法检测血清雌二醇、孕酮、泌乳素、黄体生成素和卵泡刺激素含量。结果 模型组血清雌二醇、孕酮、泌乳素、黄体生成素和卵泡刺激素水平均较空白对照组高，差异均有显著统计学意义（P<0.01）；各用药组血清性激素水平低于模型组，差异均有显著统计学意义（P<0.01）；疏肝健脾解毒方组雌二醇及黄体生成素水平低于对照药三苯氧胺组及小金丸组，差异均有统计学意义（P<0.05）；疏肝健脾解毒方组血清孕酮、卵泡刺激素、泌乳素水平高于三苯氧胺组，低于小金丸组，孕酮水平间差异有统计学意义（P<0.01），卵泡刺激素、泌乳素水平水平间差异无统计学意义（P>0.05）。结论 疏肝健脾解毒方可有效降低乳腺癌癌前病变肝郁证大鼠模型血清性激素水平。

Effect of Shugan Jianpi Jiedu Decoction on Serum Sex Hormone Levels of Breast Precancerous Lesion in Liver Depression Model Rats

Objective To study the effect of Shugan Jianpi Jiedu decoction on serum sex hormone levels of breast precancerous lesion in liver depression model rats. Methods The breast precancerous lesion of liver depression rat models were prepared by intragastric administration of two methyl benzene anthracene and tail clamping method. The rats were randomly divided into model group, Shugan Jianpi Jiedu decoction group, tamoxifen group, Xiaojinwan group, and blank control group. The levels of estrogen (E2), progesterone (P), prolactin PRL, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were measured by radioimmunoassay after 4 weeks medication respectively. Results The levels of E2, P, PRL, FSH and LH in the model group were higher than those in the blank group, the differences were statistically significant (P<0.01). The serum sex hormone levels in the treatment groups were lower than those in the model group, the differences were statistically significant (P<0.01). The estradiol and luteinizing hormone levels in Shugan Jianpi Jiedu decoction group were lower than those in the control groups, the differences were statistically significant (P<0.05). The P, FSH and PRL levels in Shugan Jianpi Jiedu decoction group were higher than tamoxifen group, lower than Xiaojinwan group, there was significant difference between the levels of P (P<0.01), there was no significant difference in the levels of FSH and PRL (P>0.05). Conclusion Shugan Jianpi Jiedu decoction could effectively reduce serum sex hormone levels of breast precancerous lesion in liver depression model rast.