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| 灰背杜鹃菌根真菌的分离与鉴定及DGGE的应用 | |
| 引用本文： | 刘振华,李潞滨,童方平,姚娜.灰背杜鹃菌根真菌的分离与鉴定及DGGE的应用[J].湖南林业科技,2012,39(4):16-20. |
| 作者姓名： | 刘振华 李潞滨 童方平 姚娜 |
| 作者单位： | 1. 湖南省林业科学院,湖南长沙410004;中国林业科学研究院林业研究所,北京100091 2. 中国林业科学研究院林业研究所,北京,100091 3. 湖南省林业科学院,湖南长沙,410004 |
| 基金项目： | 中央级公益性科研院所专项资金项目（RIF2010—16） |
| 摘 要： | 应用传统的培养方法分离菌根真菌,应用ITS rDNA分子生物学手段,进行了菌株的分类鉴定,并结合DGGE(变性梯度凝胶电泳)技术对菌根真菌进行检测。传统培养得到了2种与杜鹃花菌根真菌亲缘关系十分密切的真菌,分别为Phialocephala fortinii和Epacris pulchella root associated fungus。DGGE(变性梯度凝胶电泳)技术检测到1种菌根真菌为Rhizoscyphus ericae。 |
| 关 键 词： | 灰背杜鹃 菌根真菌 DGGE 分离 鉴定 |
Isolation and identification of mycorrhizal fungi from Rhododendron hippophaeoides and application of DGGE |
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| LIU Zhenhua , LI Lubin , TONG Fangping , YAO Na.Isolation and identification of mycorrhizal fungi from Rhododendron hippophaeoides and application of DGGE[J].Hunan Forestry Science & Technology,2012,39(4):16-20. | |
| Authors: | LIU Zhenhua LI Lubin TONG Fangping YAO Na |
| Institution: | 1. Hunan Academy of Forestry, Changsha 410004, China; Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China) |
| Abstract: | In order to understand the mycorrhizal fungi diversity of Rhododendron hippophaeoides, the mycorrhizal fungi was isolated from Rhododendron hippophaeoides, and then it was identified by both traditional classification method of morphology and rDNA ITS sequence analysis of molecular biology techniques. Two kinds of mycorrhizal strains were isolated by the traditional culture method, re- spectively： Phialocephala fortinii and Epacris pulchena root associated fungus. On the other hand, Rhizoscsphus er/cae was isolated and identified by Denaturing Gradient Gel Electrephoresis （DGGE） technique. |
| Keywords: | Rhododendron hippophaeoides mycorrhizal fungi DGGE isolation identification |
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