溶菌酶提取工艺及其在山核桃乳中的应用研究

[目的]研究制备溶菌酶的最佳工艺条件及其在山核桃乳中的应用。[方法]以鸡蛋清为原料,通过阳离子交换树脂法提取溶菌酶,采用正交试验确定制备溶菌酶的最适工艺条件,并将溶菌酶与甘氨酸和NaCl复配后作为防腐剂应用于脱脂山核桃乳饮料中。[结果]通过正交试验确定的最佳工艺条件为:蛋清树脂比5:1、pH值7.0、搅拌吸附4h,用1.0mol/LNaCl洗脱,洗脱液经超滤、浓缩后冷冻干燥得到溶菌酶成品。该试验最终酶收率为89.78%,酶活力为14817U/mg。将0.05%溶菌酶与0.30%甘氨酸和0.40%NaCl进行复配用于生产脱脂山核桃乳,其抑菌效果较显著,降低了山核桃乳的杀菌强度,延长了产品货架期。[结论]该工艺操作方便,适合溶菌酶的工业化生产;溶菌酶用于生产脱脂山核桃乳效果好。

Extraction of Lysozyme and Its Application in Chinese Walnut Milk

[Objective] The aim was to study the optimum technology condition for preparing the lysozyme and its application in Chinese walnut milk.[Method] With the chicken egg white as the raw material,the lysozyme was extracted by the cation exchange resin method.The optimum technology condition for preparing the lysozyme was determined through the orthogonal experiment and the compound of lysozyme with glycin and NaCl was used as the preservative and was applied in beverage of Chinese walnut milk.[Result] The optim...