| 二乙烯亚胺对猪细小病毒的灭活作用 |
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| 引用本文： | 傅元华,刘俊斌,钱钟,李玉和,王绍君. 二乙烯亚胺对猪细小病毒的灭活作用[J]. 中国动物传染病学报, 2012, 0(1): 73-76 |
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| 作者姓名： | 傅元华 刘俊斌 钱钟 李玉和 王绍君 |
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| 作者单位： | 中国农业科学院家禽研究所;扬州优邦生物制药有限公司 |
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| 摘 要： | 使用新型灭活剂二乙烯亚胺(binary ethylenimine,BEI)对猪细小病毒(Porcine parvovirus,PPV)进行了灭活试验,通过ST传代细胞接种法观察病毒灭活后是否出现细胞病变,并结合血凝试验检测灭活效果,确定最佳灭活方法。用3～5日龄乳鼠检测BEI灭活后的病毒培养物和相应制备疫苗的安全性,并用豚鼠检测该灭活工艺制备疫苗的效果,与传统甲醛灭活进行了比较。结果显示,终浓度为1‰的BEI在32℃情况下经20 h即可彻底灭活PPV病毒;BEI灭活的病毒制备的疫苗免疫豚鼠较甲醛灭活病毒产生较高的血凝抑制抗体。
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| 关 键 词： | 猪细小病毒 灭活 二乙烯亚胺 抗原性 |
INACTIVATION OF PORCINE PARVOVIRUS WITH BINARY ETHYLENIMINE |
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| FU Yuan-hua,LIU Jun-bin,QIAN Zhong,LI Yu-he,WANG Shao-jun. INACTIVATION OF PORCINE PARVOVIRUS WITH BINARY ETHYLENIMINE[J]. Chinese Journal of Animal Infectious Diseases, 2012, 0(1): 73-76 |
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| Authors: | FU Yuan-hua LIU Jun-bin QIAN Zhong LI Yu-he WANG Shao-jun |
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| Affiliation: | 1.Poultry Institute,CAAS,Yangzhou 225003,China;2.Yangzhou Youbang Pharmaceutical Co.,Ltd,Yangzhou225008,China) |
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| Abstract: | The present study investigated the inactivation effect of ethylenimine(BEI) on Porcine parvovirus(PPV).The assurance for BEI inactivation was performed by passaging inactivated PPV in ST cell cultures and residual viruses were examined with hemagglutination(HA) test.The inactivated virus preparation was administered into 3-5 days old suckling mice to examine the safety and into guinea pigs to evaluate the immunogenicity.Porcine parvovirus was completely inactivated with 0.1% BEI for 20 hours at 32 ℃.The HI antibody titers of guinea pigs immunized with BEI inactivated PPV was significantly higher than those immunized with formaldehyde inactivated vaccine. |
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| Keywords: | Porcine parvovirus(PPV) inactivation binary ethylenimine(BEI) immunogenicity |
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