| Studies on some characteristics of nitrate reductase from sugar beet （Beta vulgaris L.）leaves |
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| 引用本文： | LiWenhua YanGuiping 等. Studies on some characteristics of nitrate reductase from sugar beet （Beta vulgaris L.）leaves[J]. 东北农业大学学报(英文版), 1994, 1(1): 20-25 |
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| 作者姓名： | LiWenhua YanGuiping 等 |
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| 作者单位： | DepartmentofAgronomy,NortheastAgriculturaluniversity,Harbin150030,P.R.China |
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| 摘 要： | Some characteristics of nitrate reductase from sugar beet leaves shown in this paper were as follows:The nitrate reductase from sugar beet leaves required NADH as an electron donor.Accordingly,the nitrate reductase was classified as NADH-dependent(E.C.1.6.61).The Km value of the nitrate reductase for NADH and NO3^- were 0.86m mol and 0.18μ mol respectively.The optimum pH in reaction mixture solution for nitrate reduction activity was 7.5.The effect of variable concentrations of inorganic phosphorus in the reaction buffer on nitrate reductase activity was investigated.When the inorganic phosphorus concentration was below 35m mol,the nitrate reductase activity was increased with increase of inorganic phosphorus concentration.Conversely,when the inorganic phosphorus concentration was over 35m mol,the nitrate reductase activity was inhibited.The nitrate reductase activity assayed in vitro was 3.2 and 5.6times of that assayed in vivo under the condition of exogenous and endogenous ground substance respectively.
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| 关 键 词： | 甜菜叶片 硝酸还原酶 氮利用 电子供体 最佳pH Km |
Studies on some characteristics of nitrate reductase from sugar beet (Beta vulgaris L.)leaves |
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| Li Wenhua,Yan Gulping,Ma Fengming,Gao Jiguo. Studies on some characteristics of nitrate reductase from sugar beet (Beta vulgaris L.)leaves[J]. Journal of Northeast Agricultural University, 1994, 1(1): 20-25 |
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| Authors: | Li Wenhua Yan Gulping Ma Fengming Gao Jiguo |
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| Abstract: | |
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| Keywords: | Sugar beet Nitrate reductase Characteristics |
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