| 尖萼耧斗菜外植体消毒及愈伤组织诱导的研究(摘要)(英文) |
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| 引用本文： | 王非,姜思佳,李忠才,刘晓东.尖萼耧斗菜外植体消毒及愈伤组织诱导的研究(摘要)(英文)[J].Agricultural Science&Technology,2010(7). |
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| 作者姓名： | 王非 姜思佳 李忠才 刘晓东 |
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| 作者单位： | 东北林业大学园林学院;黑龙江省林业科学院林业科学研究所; |
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| 基金项目： | Supported by the Fundamental Research Funds for the Central Universities(DL09BA16); Science Research Foundation for Young Scientists of northeast Forestry University(09047 );Graduate Project Technology Innovation Funds of Northeast Forestry University~~ |
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| 摘 要： | 目的]研究尖萼耧斗菜外植体消毒及愈伤组织的诱导。方法]采用不同消毒剂种类和浓度,对尖萼耧斗菜种子进行消毒处理,研究其污染率和污染速率,寻找建立无菌苗培养系最适宜的方法;以尖萼耧斗菜种子获得的无菌苗的根、茎、叶片为外植体,通过在MS基本培养基中附加不同激素配比,筛选最佳外植体和最佳培养基配方。结果]外植体材料最适宜的灭菌处理方法为:先将外植体在75%酒精中浸30s,用无菌水冲洗5遍,再将其用0.2%升汞液浸泡2min,再用无菌水冲洗5遍。该消毒方法使污染率最低、发芽率最高,并且获得的无菌苗长势最好;诱导尖萼耧斗菜胚性愈伤组织的最佳外植体为幼根;诱导胚性愈伤组织的最佳培养基为MS0.6mg/L2,4-D+0.5mg/L6-BA。结论]该研究为尖萼耧斗菜的大规模生产提供了技术支持,并为其药用和观赏价值的最大发挥作出了贡献。
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| 关 键 词： | 尖萼耧斗菜 消毒 愈伤组织 外植体 |
Study on Explants Sterilization and Callus Induction of Aquilegia oxysepala |
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| WANG Fei,JIANG Si-jia,LI Zhong-cai,LIU Xiao-dong.College of L,scape Architecture,Northeast Forestry University,Harbin ,.Heilongjiang Academy of Forestry Science.Study on Explants Sterilization and Callus Induction of Aquilegia oxysepala[J].农业科学与技术(英文版),2010(7). |
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| Authors: | WANG Fei JIANG Si-jia LI Zhong-cai LIU Xiao-dongCollege of L scape Architecture Northeast Forestry University Harbin Heilongjiang Academy of Forestry Science |
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| Institution: | WANG Fei1,2,JIANG Si-jia1,LI Zhong-cai1,LIU Xiao-dong11.College of Landscape Architecture,Northeast Forestry University,Harbin 150040,2.Heilongjiang Academy of Forestry Science |
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| Abstract: | Objective] The aim was to study the explants sterilization and callus induction of Aquilegia oxysepala.Method] the seeds of Aquilegia oxysepala were sterilized by different kinds and concentrations of disinfectants,and the pollution rate and pollution speed were investigated so as to find the best way to build sterile seedling setup.Taking the roots,stem segments and leaves of the sterile seedlings from Aquilegia oxysepala seeds as explants,the optimum explants and medium were screened by adding MS basic ... |
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| Keywords: | Aquilegia oxysepala Sterilization Callus Explants |
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