| 平菇子实体分化发育相关基因片段的克隆 |
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| 引用本文： | 马爱民 关海山. 平菇子实体分化发育相关基因片段的克隆[J]. 华中农业大学学报, 2001, 20(4): 299-302 |
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| 作者姓名： | 马爱民 关海山 |
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| 作者单位： | 1. 华中农业大学植物保护系
2. 香港中文大学生物系 |
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| 基金项目： | 国家自然科学基金资助项目 ( 39770 5 30 ) |
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| 摘 要： | 采用RAP－PCR差异显示技术对平菇菌丝体及原基、菇蕾和成熟子实体进行研究，获得了在原基阶段差异表达的3条cDNA片段POD1、POD2与POD3，经过回收、重扩增、克隆与鉴定后进行测序分析，初步的结果表明3条差异片段的长度分别为745bp，482bp和477bp。同时，国际联网检查查明片段POD3为HSP70基因片段。
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| 关 键 词： | 分化发育相关基因 HSP70 平菇子实体 菌丝体 RAP－PCR差异显示技术 分子生物学技术 克隆 银染法 |
| 修稿时间： | 2001-03-05 |
Cloning of Differentiation and Development Related Gene Fragments in Pleurotus ostreatus |
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| Ma Aimin Guan Haishan. Cloning of Differentiation and Development Related Gene Fragments in Pleurotus ostreatus[J]. Journal of Huazhong Agricultural University, 2001, 20(4): 299-302 |
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| Authors: | Ma Aimin Guan Haishan |
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| Affiliation: | Ma Aimin 1) Guan Haishan 2) |
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| Abstract: | RAP PCR differential display technique was employed to isolate differentially expressed cDNA fragments from mycelium, primodium, young and mature fruit body in Pleurotus ostreatus . Three differentially expressed fragments POD1,POD2,POD3 in primordium were obtained. Their nucleotide sequences were determined and analyzed by Internet BLASTX+BEAUTY searching after these fragments were eluted, reamplified , cloned and identified. Preliminary results showed that the lengths of POD1,POD2,POD3 were 745 bp,482 bp,477 bp respectively. Meanwhile, POD3 was the HSP70 gene fragment. |
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| Keywords: | Pleurotus ostreatus differential display RAP PCR differentiation and development related gene HSP70 |
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