| 不同倍性金鱼草基因组DNA |
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| 引用本文： | 吴福川,胡秀,郑思乡.不同倍性金鱼草基因组DNA[J].云南农业大学学报,2005,20(4):482-485. |
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| 作者姓名： | 吴福川 胡秀 郑思乡 |
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| 作者单位： | 云南农业大学园林园艺学院，西南林学院，云南农业大学园林园艺学院 云南昆明650201，云南昆明650224，云南昆明650201云南玉溪雷森科技有限公司，云南玉溪650031 |
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| 摘 要： | 利用25个10碱基的引物，对金鱼草3个不同花色品系的二倍体及其同源四倍体基因组DNA进行随机扩增（RAPD）。结果表明：二倍体与其同源四倍体金鱼草指纹图谱的差异，随不同花色品系及不同引物而变化；四倍体金鱼草在诱导过程中除了染色体数目加倍外，基因组DNA的核苷酸碱基序列也可能改变，这为培育四倍体金鱼草新品种提供了丰富的育种资源。指纹图谱的建立为金鱼草新品种的鉴定提供分子水平的依据，为新品种保护奠定初步基础。
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| 关 键 词： | 金鱼草 RAPD 多倍体 指纹图谱 |
| 文章编号： | 1004-390X(2005)04-0482-04 |
| 收稿时间： | 2005-1-10 |
Study on Randomly Amplified Polymorphic Genome DNA of Diploid and Tetraploid Antirrihinum majus |
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| WU Fu-chuan,HU Xiu,ZHENG Si-xiang.Study on Randomly Amplified Polymorphic Genome DNA of Diploid and Tetraploid Antirrihinum majus[J].Journal of Yunnan Agricultural University,2005,20(4):482-485. |
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| Authors: | WU Fu-chuan HU Xiu ZHENG Si-xiang |
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| Institution: | WU Fu-chuan~1,HU Xiu~2,ZHENG Si-xiang~ |
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| Abstract: | With 25 10-basic primers, the genomes DNA of three groupes of diploid and tetraploid Antirrhinum majus were randomly amplified. The results showed the fingerprinting of diploid Antirrhinum majus and that of its autotatraploid were different with cultivars and primers; The DNA sequences were changed besides polyploided during polyploided inducement on Antirrhinum majus. So the abundant germresource was offered for breeding new cultivars. The fingerprinting can offer molecular evidence for identifying new cultivars of Antirrhinum majus and establish the basis for protecting new cultivars. |
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| Keywords: | Antirrhinum majus RAPD polyploid fingerprinting |
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