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| 树舌多糖的分离纯化、分子量分布及单糖组成研究 | |
| 引用本文： | 焦连庆,于敏,焦莹,刘大有. 树舌多糖的分离纯化、分子量分布及单糖组成研究[J]. 特产研究, 2009, 31(4): 59-60 |
| 作者姓名： | 焦连庆 于敏 焦莹 刘大有 |
| 作者单位： | 1. 吉林省中医药科学院,吉林,长春,130021;长春中医药大学,吉林,长春,130117 2. 吉林省中医药科学院,吉林,长春,130021 3. 长春中医药大学,吉林,长春,130117 |
| 摘 要： | 目的分离、纯化树舌总多糖,获得均一多糖。对均一多糖的分子量、重均分子量及组成糖进行分析。方法分离纯化采用DEAE离子交换色谱法;高效液相色谱法、示差折光检测器测定多糖组分的重均分子量（Mw）;气-质联用法测定单糖组成。结果得到组分Ⅰ、组分Ⅱ、组分Ⅲ三种组分。其分子量分布及单糖组成不同,含有8种单糖,组分Ⅰ、Ⅲ主要由葡萄糖组成。 |
| 关 键 词： | 树舌多糖 分离纯化 分子量分布 单糖组成 |
Studies on the Isolation, Purification, Molecular weight and Composition of polysaccharides from Ganoderma applanatum |
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| JIAO Lian-qing,YU min,JIAO Ying,LIU Da-you. Studies on the Isolation, Purification, Molecular weight and Composition of polysaccharides from Ganoderma applanatum[J]. Special Wild Economic Animal and Plant Research, 2009, 31(4): 59-60 | |
| Authors: | JIAO Lian-qing YU min JIAO Ying LIU Da-you |
| Affiliation: | JIAO Lian-qing,YU min,JIAO ying,LIU Da-you(1.Jilin Academy of Traditional Chinese Medicine Sciences,Changchun 130021,China;2.Changchun University of Traditional Chinese Medicine,Changchun 130117,China) |
| Abstract: | Object：To isolate and purify the total Ganoderma applanatum Polysaccharides,obtain homogeneous polysaccharide,analyze the molecular weight and the weight-average molecular weight and composition of Ganoderma applanatum polysaccharides.Methods：Gap were isolated and purified by DEAE 52 Ion-exchanged and sephadexG10 column chromatography;Molecular weight and Mr were determined by HPLC with RI detector;composition of monosaccharide were determined by GC.Results：Ganoderma applanatum polysaccharides was composed of three polysaccharide constitutes with different molecular weight distribution;and eight kinds of monosaccharide,GapsⅠ、GapsⅢmainly composed of D-glucose. |
| Keywords: | Ganoderma applanatum Polysaccharides（Gaps） Isolation and Purification Distribution of molecular weight Monosaccharide Composition |
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