蚕的细胞凋亡相关基因及其检测技术

家蚕细胞凋亡是家蚕细胞的主动死亡过程,在家蚕细胞的发育和疾病的发生中起重要的作用,同时也是一种抵御病毒感染的天然性保护机制。而病毒也在长期进化过程中获得了适应性调节细胞凋亡的能力,或通过在病毒复制早期表达凋亡抑制产物,以阻止细胞凋亡达到繁殖子代病毒的目的,或通过晚期表达凋亡诱导物诱导细胞凋亡,以快速有效地释放子代病毒。根据细胞凋亡的典型特征,已经发展出ELISA法,流式细胞仪法等分子生物学检测方法,为更深入地了解细胞凋亡的机制打下了坚实的基础。

Apoptosis Cell of Silkworm and its Detection Techniques

Silkworm's Apoptotic is the active death process of BmN cells, which plays an important role in the development of BmN cells and occurrence of disease, and is also an innate protective mechanism of the host against viral infection, and many virus have adapted to the host's apoptotic response by encoding factors directly inhibiting the apoptotic cascade at early infection phase, or by inducing host cell apoptosis at late infection phase for efficient progeny virus release. According to some typical characteristic of apoptosis, we have already developed some molecular biological methods such as, ELISA assay and flow cytometry assay, which makes the solid foundation for further understanding the mechanism of apoptosis.