海洋真菌HZ-01菌株产几丁质酶的研究

目的]探讨海洋真菌HZ-01菌株产几丁质酶的培养条件和部分性质。方法]从海洋环境中筛选出1株几丁质酶高产真菌HZ-01,以该菌株为材料,进行不同碳源、氮源以及温度对菌株发酵产生几丁质酶的影响试验,研究几丁质酶的培养条件。将粗酶液在不同温度下保温60 min后,取样测定几丁质酶活力,分析几丁质酶的热稳定性和酸碱稳定性。结果]海洋真菌HZ-01菌株产几丁质酶的最佳碳源为几丁质,蛋白胨为最佳氮源;以1.0%的几丁质作为碳源,0.1%的蛋白胨作为氮源,温度为30℃时,其产生的几丁质酶活力最高,且随着温度的上升,该酶活力下降;该酶的酸碱稳定性较差,只在pH值6~8具有较高活性。结论]该研究为规模化生产几丁质酶初步奠定基础。

Studies on Producing Chitinase with Marine Fungi HZ-01 Strain

Objective] The purpose was to discuss the culture condition and partial characteristics for producing chitinase with marine fungi HZ-01 strain.Method] A strain of fungi HZ-01 with high yield of chitinase was screened out from marine environment,with which as material,the experiment on the influence of different carbon(C) source,nitrogen(N) source and temperature on producing chitinase with the strain through fermentation was done to study the culture condition of chitinase.After the crude enzyme solution was treated with heat preservation for 60 min under different temperatures,the samples were fetched to determine chitinase activities and analyze the heat and acidic-basic stabilities of chitinase.Result] The optimum C source for producing chitinase with marine fungi HZ-01 strain was chitin and peptone was the optimum N source.When the temperature was 30 ℃,with 1.0% chitin as C source and 0.1% peptone as N source,the produced chitinase had highest activity and the enzyme activity decreased along with the increment of temperature.The acidic-basic stability of the enzyme was worse,which only had higher activity in pH value of 6～8.Conclusion] The research laid primary foundation for scale production of chitinase.