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湄潭苔茶良种组培快繁技术初探
引用本文:周国兰,黄燕芬,赵华富,刘晓霞,何萍.湄潭苔茶良种组培快繁技术初探[J].贵州农业科学,2012,40(1):19-21.
作者姓名:周国兰  黄燕芬  赵华富  刘晓霞  何萍
作者单位:1. 贵州省茶叶研究所,贵州湄潭,564100
2. 贵州师范学院,贵州贵阳,550018
基金项目:贵州省农业科学院专项,贵州省教育厅自然科学基金重点项目
摘    要:为探索贵州茶树组培快繁技术,采用湄潭苔茶地方良种带腋芽茎段为外植体,进行不同培养基、激素和浓度配比试验。结果表明,适宜芽诱导的培养基配方为1/2MS+6-BA 2.0mg/L+NAA 0.1mg/L+VC1 000mg/L+AC 1 500mg/L,芽诱导率可达68%;适宜芽增殖的培养基配方为MS+6-BA 0.2mg/L+NAA 0.1mg/L+GA33.0mg/L。

关 键 词:茶树  组织培养  快速繁殖

Study on Rapid Propagation Technology of Good Tai Tea Variety by Tissue Culture in Meitan
ZHOU Guo lan , HUANG Yan-fen , ZHAO Hua fu , LIU Xiao-xia , HE Ping.Study on Rapid Propagation Technology of Good Tai Tea Variety by Tissue Culture in Meitan[J].Guizhou Agricultural Sciences,2012,40(1):19-21.
Authors:ZHOU Guo lan  HUANG Yan-fen  ZHAO Hua fu  LIU Xiao-xia  HE Ping
Institution:1(1.Guizhou Tea Research Institute,Guiyang,Guizhou 550006;2.Guizhou Normal College,Guiyang,Guizhou 550001,China)
Abstract:To explore the rapid propagation of Guizhou tea by tissue culture,effects of different culture,hormone and concentration on stem with axillary buds of good Tai Tea Variety were studied.The results showed that the best medium formula for bud induction was 1/2 MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+Vc 1 000 mg/L+AC1 500 mg/L,the rate of the bud induction could be up to 68%,the best medium formula for bud breeding was MS+6-BA 0.2 mg/L+NAA 0.1 mg/L+GA3 3.0 mg/L.
Keywords:tea tree  tissue culture  rapid propagation
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