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| 高效液相色谱-二极管阵列法对中药中玉米赤霉烯酮毒素的检测效果 | |
| 引用本文： | 张晓飞,杨美华,欧阳臻. 高效液相色谱-二极管阵列法对中药中玉米赤霉烯酮毒素的检测效果[J]. 贵州农业科学, 2012, 40(4): 102-106 |
| 作者姓名： | 张晓飞 杨美华 欧阳臻 |
| 作者单位： | 1. 中国医学科学院药用植物研究所,北京100193;江苏大学药学院,江苏镇江212013 2. 中国医学科学院药用植物研究所,北京,100193 3. 江苏大学药学院,江苏镇江,212013 |
| 基金项目： | 国家自然科学基金，科技部重大新药创制专项，中医药行业科研专项 |
| 摘 要： | 为建立简便易行、快速准确检测中药中玉米赤霉烯酮毒素的方法,对107个中药样品中的玉米赤霉烯酮毒素采用高效液相色谱-二极管阵列检测法进行测定。结果表明:玉米赤霉烯酮毒素在5～1 000ng/mL范围内线性关系良好,检测限(LOD)为25.7μg/kg,回收率82.4%～92.8%,RSD2.4%～8.8%。高效液相色谱-二极管阵列检测法简便快速、通用性强、结果准确可靠,可满足不同基质中药中玉米赤霉烯酮毒素检测的需要。 |
| 关 键 词： | 玉米赤霉烯酮毒素 中药 二极管阵列检测 |
Detection of Zearalenone in Traditional Chinese Medicinal Plants by HPLC-DAD |
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| ZHANG Xiao-fei , YANG Mei-hua , OU Yang-zhen. Detection of Zearalenone in Traditional Chinese Medicinal Plants by HPLC-DAD[J]. Guizhou Agricultural Sciences, 2012, 40(4): 102-106 | |
| Authors: | ZHANG Xiao-fei YANG Mei-hua OU Yang-zhen |
| Affiliation: | 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193;2.School of Pharmacy,Jiangsu University,Zhenjiang,Jiangsu 212013,China) |
| Abstract: | In order to establish a simple,quick and accurate assay for detection of Zearalenone in traditional Chinese medicine,the Zearalenone in 107 Chinese medicinal samples was detected by HPLC-DAD.The results showed that a good linear corralation was demonstrated at the Zearalenone concentration of 5~1000 ng/ml and the detection limit was 25.7 g/kg.The recoveries of analytes were from 82.4 to 98.2% while RSD was from 2.4 to 8.8%.This method could be applied to detect of Zearalenone in different traditional Chinese medicines for its high rapidness and precision. |
| Keywords: | zearalenone Chinese medicine diode array detector |
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