| 前期处理对西瓜花药培养愈伤组织诱导的影响 |
| |
| 引用本文： | 朱迎春,孙德玺,邓云,安国林,李卫华,刘君璞.前期处理对西瓜花药培养愈伤组织诱导的影响[J].中国瓜菜,2012,25(5):17-19. |
| |
| 作者姓名： | 朱迎春 孙德玺 邓云 安国林 李卫华 刘君璞 |
| |
| 作者单位： | 中国农业科学院郑州果树研究所 郑州450009 |
| |
| 基金项目： | 现代农业产业技术体系建设专项（CARS-26-14） |
| |
| 摘 要： | 以6份不同基因型西瓜材料为试材,研究了HgCl2消毒处理时间和低温预处理时间对西瓜花药培养愈伤组织诱导的影响。结果表明:用75%酒精处理30s,再用0.1%的HgCl2消毒8min时,西瓜花药污染率和褐化率均较低,可作为西瓜花药培养的主要消毒方法;在4℃预处理48、72h时,西瓜花药培养愈伤组织诱导率较高,均显著高于对照,说明4℃低温预处理可作为花蕾的一种保存方式;基因型间愈伤组织诱导率差异显著,1005愈伤组织诱导率最高。
|
| 关 键 词： | 西瓜 花药培养 低温预处理 消毒处理 愈伤组织诱导 |
Effects of Preliminary Treatment on Watermelon Anther Culture Callus Induction |
| |
| ZHU Ying-chun
,
SUN De-xi
,
DENG Yun
,
AN Guo-lin
,
LI Wei-hua
,
LIU Jun-pu.Effects of Preliminary Treatment on Watermelon Anther Culture Callus Induction[J].China Cucurbits And Vegetables,2012,25(5):17-19. |
| |
| Authors: | ZHU Ying-chun SUN De-xi DENG Yun AN Guo-lin LI Wei-hua LIU Jun-pu |
| |
| Institution: | ( Zhengzhou Fruit Research Institute, CAAS, Zhengzhou, Henan, 450009, China) |
| |
| Abstract: | The anthers of six watermelon genotypes were used as test materials to investigate the factors of the time of anther low temperature pretreatment arid HgC12 disinfection on watermelon anther culture callus induction. The results showed that the callus induction ratios were significantly different among tested watermelon genotypes, 1005 had the highest callus induction rate;The pretreatment of anthers at 4 ℃for 48 h and 72 h can induce higher callus rate,the callus induction ratios significantly higher than those of the control;When the time of the 75% alcohol sterilization was 30 s and 0.1% HgC12 disinfection was 8 min,the pollution rate and browning rate of the watermelon anther were all lower,that can be used as main disinfection method of watermelon anther culture. |
| |
| Keywords: | Watermelon Anther culture Low temperature pretreatment HgCI2 disinfection Callus induction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
