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西瓜甜瓜蔓枯病病菌差异性初步研究
引用本文:胡凤云,莫贱友,韦继光,郭堂勋,黄穗萍,潘朝勃.西瓜甜瓜蔓枯病病菌差异性初步研究[J].中国西瓜甜瓜,2012(6):9-12.
作者姓名:胡凤云  莫贱友  韦继光  郭堂勋  黄穗萍  潘朝勃
作者单位:[1]广西药用植物园,南宁530023 [2]广西壮族自治区农业科学院植物保护研究所,南宁530007 [3]广西大学农学院,南宁530003
基金项目:南宁市科技计划项目(201003035B); 南宁市青秀区科技计划项目(2010N25,20090211B); 2011年广西研究生教育创新计划项目(T32611)
摘    要:初步研究了西、甜瓜蔓枯病病菌差异性,为深入了解西、甜瓜蔓枯病病原提供理论依据。采用离体叶片接种法对 19 个西、甜瓜蔓枯病菌株进行致病力差异性研究,并对不同菌株核糖体基因内转录区序列(rDNA-ITS)比较分析。结果发现:不同西、甜瓜蔓枯病菌株致病力存在显著性差异,病情指数为 85.11~4.58;但不同菌株间的 ITS 序列比较差别不大,保守程度高,菌株间的分化和变异不明显。

关 键 词:西瓜  甜瓜  蔓枯病  病菌差异性

Preliminary Study of the Variation of Watermelon and Melon Gummy Stem Blight Pathogen
HU Feng-yun,MO Jian-you,WEI Ji-guang,GUO Tang-xun,HUANG Sui-ping,PAN Chao-bo.Preliminary Study of the Variation of Watermelon and Melon Gummy Stem Blight Pathogen[J].China Watermelon and Muskmelon,2012(6):9-12.
Authors:HU Feng-yun  MO Jian-you  WEI Ji-guang  GUO Tang-xun  HUANG Sui-ping  PAN Chao-bo
Institution:1.Guangxi Botanical Garden of Medicinal Plants,Nanning,Guangxi,530023,China;2.Plant Protection Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi,530007,China;3.Agricultural College,Guangxi University,Nanning,Guangxi,530003,China)
Abstract:The knowledge of pathogen variation is needed for the research of watermelon and melon gummy stem blight.The pathogenicity of 19 strains of Didymella bryoniae was assayed using detached leaf inoculation.These strains were further analyzed for their sequence at ITS region of rDNA(rDNA-ITS).The pathogenicity of these strains was significantly different with disease indexes 85.11-4.58 on same watermelon variety.However,the sequences at ITS region were much conserved among these strains.
Keywords:Watermelon  Melon  Didymella bryoniae  Pathogen variance
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