| 鸡大肠杆菌计数方法研究 |
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| 引用本文： | 陶海静. 鸡大肠杆菌计数方法研究[J]. 安徽农业科学, 2007, 35(27): 8504-8504,8516 |
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| 作者姓名： | 陶海静 |
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| 作者单位： | 郑州牧业工程高等专科学校生物工程系,河南郑州,450011 |
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| 摘 要： | 寻求一种简单、快捷的大肠杆菌计数方法。采用分光光度计测定经鉴定的大肠杆菌悬液的OD值,同时通过直接涂片计数法计算大肠杆菌数。细菌悬液经适当浓度稀释后OD值在0.1~1.0范围内,细菌悬液的OD值与活菌数之间成直线关系。利用OD值测定大肠杆菌数目是一种简单、快捷的细菌计数方法。
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| 关 键 词： | 大肠杆菌 OD值 计数法 |
| 文章编号： | 0517-6611（2007）27-08504-01 |
| 修稿时间： | 2007-05-14 |
Study on the Counting Method of E. coli in Chicken |
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| TAO Hai-jing. Study on the Counting Method of E. coli in Chicken[J]. Journal of Anhui Agricultural Sciences, 2007, 35(27): 8504-8504,8516 |
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| Authors: | TAO Hai-jing |
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| Affiliation: | Department of Biological Engineering, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou,Henan 450011 |
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| Abstract: | The objective of the research was to seek a simple and rapid counting method of E. coli. The OD value of E, eoli suspension that was identified was determined by speetmphotometer and E, coli number was calculated by direct smear counting method. When the OD value of bacteria suspension diluted by appropriate concentration was in the range of 0. 1 - 1.0, the OD value of bacteria suspension and living bacteria number were in linear relation. Determining E. coli number by using OD value was a simple and rapid bacteria counting method. |
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| Keywords: | E.coli OD value Counting method |
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