| 旱柳Q106组织培养及快繁体系的建立 |
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| 引用本文： | 余如刚,杜雪玲,夏阳,梁慧敏,李阳春.旱柳Q106组织培养及快繁体系的建立[J].草原与草坪,2005(5):57-59. |
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| 作者姓名： | 余如刚 杜雪玲 夏阳 梁慧敏 李阳春 |
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| 作者单位： | [1]安徽淮北煤炭师范学院,安徽淮北235000 [2]甘肃农业大学草业学院,甘肃兰州730070 [3]山东省林业科学研究院,山东济南250014 |
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| 基金项目： | 山东省科技发展基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 以旱柳(Salixmatsudana)Q106春季萌生嫩枝为外植体,建立了“以芽繁芽”的组织培养快繁体系。丛生芽培养基为MS+BA0.4mg/L+NAA0.2mg/L。继代培养基为MS+BA0.4mg/L+NAA0.2mg/L和BA0.4mg/L+NAA0.4mg/L;MS+BA0.4mg/L+NAA0.2mg/L和BA0.4mg/L+IBA0.2mg/L交替使用。最佳生根培养基为MS+GA30.2mg/L+NAA0.5mg/L。
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| 关 键 词： | 旱柳Q106 组织培养 激素 |
| 文章编号： | 1009-5500（2005）05-0057-03 |
| 收稿时间： | 2004-06-09 |
| 修稿时间： | 2004年6月9日 |
Tissue culture and rapid propagation of Salix matsudana Q106 in vitro |
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| YU Ru-gang.Tissue culture and rapid propagation of Salix matsudana Q106 in vitro[J].Grassland and Turf,2005(5):57-59. |
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| Authors: | YU Ru-gang |
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| Institution: | 1. Huaibei Coal Industry Normal College, Huaibei 235000, China; 2. Pratacultural College,Gansu Agricultural University,Lanzhou 730070, China ;3. Shandong Provincial Academy of Forestry, Jinan 250014, China |
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| Abstract: | Rapid propagation system of Salix matsudana Q106 was established by using one axillary bud stem as explants which derived from internodal sections of 1-year-old Salix matsudanaQ106.The results obtained as follows: the axillary bud can obtain better multiplication on MS +(0.4)(mg/L) BA +(0.2)(mg/L) NAA culture,while developed plants were sub-cultured on MS+(0.4)(mg/L) BA+(0.2)(mg/L) NAA and(0.4)(mg/L) BA+(0.4)(mg/L) NAA or MS+(0.4)(mg/L) BA+(0.2)(mg/L) NAA alternate with(0.4)(mg/L) BA+(0.2)(mg/L) IBA culture.The optimum culture for rooting is MS+(0.2)(mg/L) GA_(3)+0.5 mg/L NAA. |
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| Keywords: | Salix matsudana Q106 tissue culture phytohormone |
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