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| 内蒙古绒山羊Homeobox基因片段的克隆与序列分析 | |
| 引用本文： | 张燕军,尹俊,梁永厚,李荣,刘弈弈,张俊霞,席海燕,刘志红,李金泉. 内蒙古绒山羊Homeobox基因片段的克隆与序列分析[J]. 江西农业大学学报, 2006, 28(6): 919-922 |
| 作者姓名： | 张燕军 尹俊 梁永厚 李荣 刘弈弈 张俊霞 席海燕 刘志红 李金泉 |
| 作者单位： | 1. 内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018 2. 内蒙古农业大学,生物工程学院,内蒙古,呼和浩特,010018 3. 内蒙古农业大学 动物科学与医学学院,内蒙古,呼和浩特,010018;内蒙古河套大学 动物科学系,内蒙古,临河,015000 |
| 摘 要： | 根据已公布的Hom eobox基因氨基酸序列保守区设计简并引物,利用PCR技术从内蒙古绒山羊血液基因组DNA中扩增Hom eobox基因家族成员。目的基因片段纯化后连接到pGEM-T载体上,经鉴定得到重组质粒。将110个重组质粒进行测序,测序结果通过与GenBank中序列比对分析,确定86条序列为Hom eobox基因,得到14个Hom eobox基因家族成员:Hoxa4,Hoxa5,Hoxa6,Hoxa7,Hoxb1,Hoxb2,Hoxb3,Hoxb6,Hoxb7,Hoxc6,Hoxd1,Hoxd3,Gbx1,Gbx2。每个基因片段由117个核苷酸组成,编码39个氨基酸。氨基酸序列非常保守,和其他物种的同源性非常高。 |
| 关 键 词： | Homeobox基因 简并引物 绒山羊 |
| 文章编号： | 1000-2286（2006）06-0919-04 |
| 收稿时间： | 2006-06-19 |
| 修稿时间： | 2006-06-19 |
Cloning and sequence analysis of homeobox gene fragments of Inner Mongolian cashmere goat |
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| ZHANG Yan-jun,YIN Jun,LIANG Yong-hou,LI Rong,LIU Yi-yi,ZHANG Jun-xia,XI Hai-yan,LIU Zhi-hong,LI Jin-quan. Cloning and sequence analysis of homeobox gene fragments of Inner Mongolian cashmere goat[J]. Acta Agriculturae Universitis Jiangxiensis, 2006, 28(6): 919-922 | |
| Authors: | ZHANG Yan-jun YIN Jun LIANG Yong-hou LI Rong LIU Yi-yi ZHANG Jun-xia XI Hai-yan LIU Zhi-hong LI Jin-quan |
| Affiliation: | 1. College of Animal Science and Medical Science, Inner Mongolia Agricultural University, Huhhot 010018, China ; 2. College of Biotechnology, Inner Mongolia Agricultural University, Huhhot 010018, China ; 3. Department of Animal Science, Inner Mongolia Hetao University, Linhe 015000, China |
| Abstract: | According to the conservative amino acid sequences of announced homeobox genes,degenerate primers were designed.The homeobox gene fragments of Inner Mongolian cashmere goat were amplified by PCR.The PCR products were purified and then cloned into pGEM-T vector.One hundred and ten 110 recombinant plasmids were sequenced.By blasting with database of GenBank,86 sequences were homeobox gene and classed into 14 kinds:Hoxa4,Hoxa5,Hoxa6,Hoxa7,Hoxb1,Hoxb2,Hoxb3,Hoxb6,Hoxb7,Hoxc6,Hoxd1,Hoxd3,Gbx1and Gbx2.Every gene fragment was composed of 117 nucleotides which encoded a high conservative 39 amino acid peptide and the amino acid sequence had a high homology compared with other species. |
| Keywords: | Homeobox gene degenerate primer cashmere goat |
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