| 新城疫病毒分离株融合蛋白基因的遗传变异分析 |
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| 引用本文： | 魏玉荣,刘丽娜,沈国顺,吴高峰. 新城疫病毒分离株融合蛋白基因的遗传变异分析[J]. 畜禽业, 2006, 0(2): 22-25 |
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| 作者姓名： | 魏玉荣 刘丽娜 沈国顺 吴高峰 |
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| 作者单位： | 沈阳农业大学畜牧兽医学院,辽宁,沈阳,110161 |
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| 摘 要： | 根据GenBank中报道的NDV融合蛋白基因(F)序列,设计了一对特异性引物,用该引物对所分离的LN-SN株进行了RT-PCR扩增;将扩增得到的PCR片段纯化后与pGEM-T连接得到重组质粒pGEM-F,用于核苷酸序列测定。结果该基因ORF长1662bp,编码553个氨基酸;将LN-SN毒株与GenBank中已报道的NDV毒株进行比较,F基因核苷酸序列的同源性在83.1%～98.9%之间,推导的F蛋白氨基酸序列的同源性在88.1%～99.5%之间。
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| 关 键 词： | 新城疫病毒 融合蛋白基因 遗传变异 |
| 文章编号： | 1008-0414(2006)01(下)-0022-04 |
| 修稿时间： | 2005-10-24 |
Heredity And VariationAnalysis of Fusion Protein Gene of Newcastle Disease Virus |
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| WEI Yu-rong,LIU Li-na,SHEN Guo-shun,WU Gao-feng. Heredity And VariationAnalysis of Fusion Protein Gene of Newcastle Disease Virus[J]. Livestock and Poultry Industry, 2006, 0(2): 22-25 |
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| Authors: | WEI Yu-rong LIU Li-na SHEN Guo-shun WU Gao-feng |
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| Abstract: | According to fusion protein gene sequence of Newcastle Disease virus strain reported by GenBank database,a pair of specific primers was designed and used to amplify F gene of LN-SN strain.The positive PCR product was purified and ligatured with pGEM-T.The correct positive recombinant was used for sequencing.The open reading frame length of F gene of LN-SN strain was 1662bp and encoded 553 amino acids.The homology of nucleic acid and amino acid among the strains of NDV accessed by GenBank database ranged from 83.1 to 98.9% and from 88.1 to 99.5% ? |
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| Keywords: | Newcastle Disease virus fusion protein gene Heredity and Variation |
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