脱氢表雄酮对雄性小鼠睾丸组织发育的毒性作用

昆明种雄性小鼠32只，随机分为4组。脱氢表雄酮（DHEA）日摄取量分别为0（对照组）、20、40和60mg／kg，试验期52d。各组分别在42日龄、72日龄处死小鼠，分离睾丸，制作石蜡切片，HE染色，显微观察、摄影。结果显示，与对照组比较，42日龄时中、高剂量组的曲精小管断面变形，各时期的生精细胞和成熟精子数量均减少；高剂量组曲精小管内生精细胞层次基本消失，出现较大腔隙，间质发生不同程度的变化。72日龄时中、高剂量组的曲精小管断面变形。基膜变薄伴有断裂和间质受损增加。中剂量组出现畸形精子，高剂量组曲精小管中央空白区域增大，缺乏成熟精子。以上结果表明，中、高剂量的DHEA对生长期小鼠的睾丸组织结构发育有明显的毒性作用。

Toxic Effect of Dehydroepiandrosterone on the Testis Tissue Development of Male Mice

32 Kunming male mice were randomly divided into 4 groups,which were fed with DHEA of 0 mg/kg （control group）, 20 mg/kg（low-dose group）, 40 mg/kg （medium-dose group） and 60 mg/kg （high-dose group） every day. The experiment lasted for 52 days. The mice in every group were killed on the 42 day-old and 72 day-old respectively. The testis was picked to make into paraffin sections and observe in microscope after HE staining. The results showed that the cross-section of seminiferous tubules deformed on 42 day-old and the number of spermatogenic cells and mature spermatozoa in each period all reduced in medium-dose group and high-dose group, compared with control group. In high-dose group, the hierarchy of spermatogenic cell in seminiferous tubule basically disappeared, bigger lacuna appeared and interstitium changed to different extent. On 72 day-old, the cross-section of seminiferous tubules deformed, basement membrane became thin accompanied by fracture and damaged extent of interstitium increased in medium-dose group and high-dose group. Abnormal sperms were found in medium dose group. In high-dose group, central blank area in seminiferous tubule was enlarged and mature sperm lacked. The results suggested that medium-dose and high-dose of DHEA had obvious toxic effects on the structure development of testieular tissue in growing mice.