In vitro culture of tissues,cells and protoplasts of Trifolium alexandrinum L. (Egyptian clover) |
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Authors: | M N Barakat |
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Institution: | (1) Tissue Culture Laboratory, Department of Crop Science, Faculty of Agriculture, University of Alexandria, Egypt |
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Abstract: | Summary Callus induction derived from root, hypocotyl and cotyledon explants were investigated for four cultivars of Trifolium alexandrinum L. The Murashige & Skoog (1962) medium containing 2.0 mg/L NAA and 0.5 mg/L BAP was able to induce callus from different explants. A wide range of culture media were tested to determine the morphogenetic potential of different callus types derived from different explant types. Shoot morphogenetic development was observed with the cultivars Sakha 4 and Giza 10. Cell suspension cultures were established from hypocotyl derived callus. Methods for isolation and culture of protoplasts from cotyledons are described. |
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Keywords: | Trifolium alexandrinum Egyptian clover in vitro culture plant regeneration |
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