水稻低温发芽力的QTL定位

以珍汕97B与多年生稻种质AAV002863的DH群体(198个株系)构建了包含140个SSR标记的连锁图谱，检测了影响水稻低温发芽力性状的数量性状座位（QTL）。15℃下处理6 d，两亲本珍汕97B与多年生稻AAV002863的发芽率分别为79.7%和30.1%，DH群体间的发芽率变化在0%~100%。QTL定位分析检测到2个与低温发芽力相关的基因座，分别位于第3和第10染色体上，贡献率分别为12.6%和12.9%，增效等位基因分别来自多年生稻AAV002863和珍汕97B。上位性分析结果显示，第1与第10染色体上存在影响低温发芽力的互作位点，其互作可以提高低温发芽力，参与互作的第10染色体上的位点也具有加性主效应。

QTL Mapping of Low Temperature Germinability in Rice

A double haploid population (DH) with 198 lines, derived from anther culture of F 1 hybrid between indica line Zhenshan 97B and japonica perennial rice line AAV002863 was used to construct a linkage map with 140 SSR markers and to investigate the low temperature germinability (LTG). The germination rates of Zhenshan 97B and AAV002863 were 79.7% and 30.1%, respectively, and those of the DH population ranged from 0% to 100% at 15℃ for six days. Quantitative trait loci (QTLs) controlling low temperature germinability were identified on chromosomes 3 and 10. The percentage of observed phenotypic variance attributed to qLTG-3 and qLTG-10were 12.6% and 12.9%, respectively. Allele from Zhenshan 97B increases the LTG at the region of qLTG-3, while allele from AAV002863 increases the LTG at the region of qLTG-10. One pair of epistatic interaction were detected between loci on chromosomes 3 and 10. The main-effect QTL on chromosome 10 was also involved in epistatic interaction.