| PCR技术在植物检疫性病害鉴定中的应用 |
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| 引用本文： | 何末军,周国英,李河.PCR技术在植物检疫性病害鉴定中的应用[J].西南林学院学报,2009,29(1):89-93. |
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| 作者姓名： | 何末军 周国英 李河 |
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| 作者单位： | 中南林业科技大学,资源与环境学院,湖南,长沙,410004 |
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| 基金项目： | 国家科技支撑项目，国家林业局重点项目，湖南省教育厅科研项目，中南林业科技大学青年科研基金，中南林业科技大学级研究生科技创新基金 |
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| 摘 要： | 简述了传统PCR、实时定量PCR以及多重PCR技术的基本原理及在植物检疫中的应用，分析了目前PCR技术存在的问题和应用前景。
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| 关 键 词： | 检疫性病害 PCR 实时荧光定量PCR 多重PCR |
Application of PCR Techniques to Identification of Plant Quarantine Disease |
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| HE Mo-jun,ZHOU Guo-ying,LI He.Application of PCR Techniques to Identification of Plant Quarantine Disease[J].Journal of Southwest Forestry College,2009,29(1):89-93. |
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| Authors: | HE Mo-jun ZHOU Guo-ying LI He |
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| Institution: | (College of Resource and Environment, Central South China University of Forestry Science and Technology, Changsha Hunan 410004, China) |
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| Abstract: | The fundamental principles of PCR technologies including the traditional PCR, real-time quantitative PCR and multiplex PCR, and the application of these techniques to plant quarantine were briefly introduced. The technical problems related to PCRs and the application prospect were expounded in the paper. |
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| Keywords: | PCR |
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