物理伤害诱导白木香应激反应的生理特征

为了分析物理伤害诱导白木香应激反应特征,本研究以3年生普通白木香与易结香白木香品系为对象,采用机械打孔处理,分析其受伤害前后的激素含量、抗氧化性酶活性、酚类与萜类防御物质以及萜类前体物质乙酰辅酶A含量的变化特征,比较两类白木香受物理伤害后的应激反应特征。结果表明：机械损伤后,普通白木香与易结香白木香茉莉酸、水杨酸、乙烯含量都显著增加。除抗坏血酸氧化酶外,其他5种抗氧化性酶活性显著增强。防御物质总酚、总萜含量在普通白木香中分别增加了15.31%和9.04%,在易结香白木香中分别增加了24.63%和15.08%。乙酰辅酶A在两类白木香中也显著增加。其中,易结香白木香中茉莉酸、乙烯前体含量变化显著高于普通白木香。且易结香白木香的超氧化物歧化酶、过氧化氢酶、过氧化物酶活性变化分别是普通白木香的1.63、1.60、1.55倍。易结香白木香的总酚和总萜增量显著大于普通白木香。易结香白木香中乙酰辅酶A的增量是普通白木香的1.74倍。综上所述,白木香在受到机械损伤后应激反应物质都得到了增加,并且多数指标在易结香白木香品系中显著高于普通白木香,表明在物理伤害诱导下易结香白木香应激反应强于普通白木香。茉莉酸、乙烯、超氧化物歧化酶、过氧化氢酶、过氧化物酶、总酚、总萜以及乙酰辅酶A等应激反应的特征物质在两类白木香中表现出显著差异,可作为快速判断易结香白木香品系的有效生理指标。

Physiological Characteristics of Stress Response Induced by Physical Injury in Aquilaria sinensis

Mechanical drilling treatment was implemented for three-year-old common Aquilaria sinensis and easily induced agarwood A. Sinensis to analyze the characteristics of stress response of A. sinensis induced by physical injury. The changes of hormone content, antioxidant enzyme activity, phenolic and terpenoid defense substances, and terpenoid precursor acetyl-CoA content were analyzed before and after mechanical injury. The stress response characteristics of two kinds of A. sinensis after physical injury were compared. The contents of jasmonic acid, salicylic acid and ethylene of common Aquilaria sinensis and easily induced agarwood A. sinensis increased significantly after mechanical damage. Except for ascorbate oxidase, the activities of the other five antioxidant enzymes enhanced significantly. The contents of total phenols and total terpenes were increased by 15.31% and 9.04% in common A. sinensis, and 24.63% and 15.08% in easily induced agarwood A. sinensis respectively. Acetyl-CoA also increased significantly in both kinds of A. sinensis. Among them, the increments of jasmonic acid and ethylene precursor contents were significantly higher in easily induced agarwood A. sinensis than those in common A. sinensis. The activity changes of superoxide dismutase, catalase and peroxidase of easily induced agarwood A. sinensis were 1.63, 1.60 and 1.55 times of common A. sinensis respectively. The increments of total phenols and terpenes of easily induced agarwood A. sinensis were significantly higher than that of common A. sinensis. The increment of acetyl-CoA in easily induced agarwood A. sinensis was 1.74 times higher than that in common A. sinensis. In conclusion, stress response substances of A. sinensis increased after mechanical injury, and most indexs of them were significantly higher in easily induced agarwood A. sinensis than those in common A. sinensis. It was suggested that the stress response of easily induced agarwood Aquilaria sinensis was stronger than common Aquilaria sinensis. Jasmonic acid, ethylene, superoxide dismutase, catalase, peroxidase, total phenols, total terpenes and acetyl-CoA were significantly different between the two kinds of Aquilaria sinensis, which could be used as effective physiological indexes for rapid identification of easily induced agarwood Aquilaria sinensis.