正交设计优化中药类植物提取物组方

为制备可替代饲用抗生素的中药类复合植物提取物,首先以抑菌率、抗炎率和抗氧化IC₅₀值为评价指标,从19种中药类植物中遴选出石榴皮、桂枝、厚朴、青蒿和鸡血藤5种原料作为复合提取物组分;然后采用L₁₆(4⁵)正交试验设计优化组分不同配比,对试验结果进行极差分析,获得分别具有最佳抑菌(F_ab)、抗炎(F_ai)和抗氧化(F_ao)功能的3个组方,其组分中厚朴、鸡血藤、青蒿、桂枝和石榴皮的比例依次为7∶13∶6∶9∶1、7∶13∶4∶1∶1和3∶13∶4∶1∶7。验证试验结果显示:F_ab最小抑菌浓度为125 mg·mL^-1,F_ai抗炎IC₅₀值为0.144 mg·mL^-1,F_ao抗氧化IC₅₀值为0.222 mg·mL^-1。通过以上研究,获得兼具抑菌、抗炎和抗氧化功能的复合植物提取物最优组方3个。

Optimization of Chinese medicine plant extract compounds with orthogonal design

To prepare Chinese medicine plant extracts replacing feed antibiotics. Firstly, Grana Pericarpium, Cinnamomi Ramulus, Magnoliae officinalis cortex, Artemisiae annuae herba, Spatholobi caulis were sieved from 19 Chinese medicine plants as materials of extract compounds by examining antibacterial rate, inhibitory rateanti-inflammatory rate, and antioxidant IC₅₀ value as evaluating index. Secondly, proportions of five materials were optimized by a L₁₆ (4 ⁵) orthogonal experiment,by range analysis of results, three extract compounds with the strongest antibacterial (F_ab), anti-inflammatory (F_ai) and antioxidant (F_ao) activities were obtained. These material ratios of Magnoliae officinalis cortex, Spatholobi caulis, Artemisiae annuae herba, Cinnamomi Ramulus and Grana Pericarpium were 7∶13∶6∶9∶1 in F_ab, 7∶13∶4∶1∶1 in F_ai, and 3∶13∶4∶1∶7 in F_ao, respectively. Finally, the verifying results showed that the minimum inhibitory concentration of F_ab was 125 mg·mL^-1, the anti-inflammatory IC₅₀ value of F_ai was 0.144 mg·mL^-1, and the antioxidant IC₅₀ value of F_ao was 0.222 mg·mL^-1. In summary, the present study obtained the best three Chinese medicine extract compounds with stronger antibacterial, anti-inflammatory and antioxidant effects, respectively.