水稻稻瘟病拮抗稀有放线菌的筛选及防治效果

为获得对水稻稻瘟病菌具有良好抑菌效果的稀有放线菌,从红豆杉根际土壤样品中分离获得11株放线菌,筛选出对水稻稻瘟病菌抑菌效果最好的菌株ST7-2,抑菌率为79.88％,经形态特征、生理生化特征观察和16S rRNA、recA、gyrB、rpoB序列分析,确定菌株ST7-2为杀结节链霉菌Streptomyces tuber...

Screening and Control Effect of Rare Antagonistic Actinomycetes against Rice Blast

To screen the rare actinomycetes with good antifungal effect against the Magnaporthe oryzae. Actinomycetes were isolated from rhizosphere soil of Taxus chinensis. The results showed that a total of 11 strains of actinomycetes were isolated. Strain ST7−2 showed the best inhibitory effect on M. oryzae, and the inhibitory rate was 79.88%. ST7−2 was identified as Streptomyces tubercidicus combining 16S rRNA, recA, gyrB, rpoB sequence analysis, and the morphological, physiological, and biochemical characteristics. In addition, ST7−2 displayed good effects on Magnaporthe oryzae, Colletotrichum truncatum, Phomopsis asparagi, Ustilaginoidea virens and Mycogone perniciosa. The control effect of fermentation broth against seeding blast was 82.76% under the greenhouse, which showed no significant difference with that of 75% tricyclazole wettable powder 1700 fold. In field trials, the control effect of fermentation broth was 73.57%, which was slightly less effective than 75% tricyclazole WP. Therefore, S. tubercidicus ST7−2 is a potential biocontrol strain against rice blast, and show a wild prospect for development and application in the future.