| SSR分子标记鉴定橡胶树F1真伪杂种 |
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| 引用本文： | 李文秀,贺军军,张华林,罗萍. SSR分子标记鉴定橡胶树F1真伪杂种[J]. 热带作物学报, 2021, 42(5): 1305-1309. DOI: 10.3969/j.issn.1000-2561.2021.05.015 |
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| 作者姓名： | 李文秀 贺军军 张华林 罗萍 |
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| 作者单位： | 中国热带农业科学院湛江实验站/广东省旱作节水农业工程技术中心,广东湛江 524091;中国热带农业科学院南亚热带作物研究所,广东湛江 524013;中国热带农业科学院湛江实验站/广东省旱作节水农业工程技术中心,广东湛江 524091 |
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| 基金项目： | 国家重点研发计划项目“橡胶树抗寒高产品种改良”(2019YFD1001102);国家天然橡胶产业技术体系湛江综合试验站项目(CARS-33-GD2);海南省自然科学基金青年基金项目(318QN291) |
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| 摘 要： | 早期快速鉴定橡胶树F1杂交子代,获取真杂种对橡胶树优良品系的创育和遗传学研究具有重要的意义.以橡胶树优良品系'PR107'和'93-114'进行杂交,获得杂交组合子代,以亲本作为模板从104对SSR引物中筛选出3对多态性引物对35株杂交子代进行真实性鉴定,结果表明:35株杂交子代均鉴定为真杂种苗;筛选出的3对多态性引物...
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| 关 键 词： | 橡胶树 SSR分子标记 杂种鉴定 杂交育种 |
| 收稿时间： | 2020-06-23 |
Identification of F1 Hybrids of Hevea brasiliensis by SSR Markers |
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| LI Wenxiu,HE Junjun,ZHANG Hualin,LUO Ping. Identification of F1 Hybrids of Hevea brasiliensis by SSR Markers[J]. Chinese Journal of Tropical Crops, 2021, 42(5): 1305-1309. DOI: 10.3969/j.issn.1000-2561.2021.05.015 |
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| Authors: | LI Wenxiu HE Junjun ZHANG Hualin LUO Ping |
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| Affiliation: | 1. Zhanjiang Experimental Station, China Academy of Tropical Agricultural Sciences / Guangdong Dry Farming Water-saving Agricultural Engineering Technology Research Center, Zhanjiang, Guangdong 524091, China2. South Subtropical Crop Research Institute, China Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524013, China |
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| Abstract: | Early and rapid identification of F1 hybrid progeny of Hevea brasiliensis and acquisition of true hybrids are of great significance to the creation and genetic research of H. brasiliensis. In this paper, ‘PR107’ and ‘93-114’ were used to hybridize, and three pairs of polymorphic complementary primers were selected from 104 pairs of SSR primers. The results showed that all 35 hybrid progenies were identified as true hybrid seedlings. All the three pairs of polymorphic primers screened had high heterosis identification rate, and the reliability of the identification of genuine and false hybrids was verified by one pair of primers, which could be used as ideal SSR molecular markers for the authenticity identification of rubber tree hybrids later. |
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| Keywords: | Hevea brasiliensis SSR molecular marker hybrid identification hybrid breeding |
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