椰心叶甲肠道微生物的分离鉴定及功能分析

椰心叶甲（Brontispa longissima Gestro）是近年来危害棕榈科植物的主要入侵害虫之一,能够对多种棕榈科植物造成严重危害。本研究通过对椰心叶甲肠道共生菌进行分离培养,并鉴定其相关功能,以探究肠道共生菌对椰心叶甲环境适应性的作用。对椰心叶甲幼虫肠道共生菌进行分离培养,提取DNA以通用引物扩增鉴定后,以透明圈染色实验对纤维素、木聚糖和果胶功能进行分析。椰心叶甲幼虫肠道中分离得到22株细菌和2株真菌,其中可培养细菌的优势菌群为变形菌门（Proteobacteria）。芽孢杆菌属细菌具有降解纤维素和木聚糖的能力,而带化红球菌具有降解木聚糖的能力。椰心叶甲肠道中具有能够降解纤维素与木聚糖的微生物,并占据一定优势地位,因此椰心叶甲能够取食危害多种纤维素含量较高的棕榈科植物,而这一类肠道共生菌对于椰心叶甲入侵过程中的适应性也提供了一定的帮助。

Isolation,Identification and Functional Analysis of Intestinal Microorganisms of Brontispa longissimi Gestro

Brontispa longissima Gestro is one of the major invasive pests of Palmae in recent years, which has great damage to many Palmae plants. In this paper, we isolated and cultured the intestinal symbiotic bacteria of B. longissimi Gestro, and identified its related functions, in order to explore the role of intestinal symbiotic bacteria on the environmental adaptability of B. longissimi Gestro. The intestinal symbiotic bacteria of B. longissimi Gestro were isolated and cultured, DNA was extracted, amplified and identified with universal primers, and analyzed its function by transparent circle staining experiment. 22 culturable bacteria and 2 fungi were isolated and sequenced from the larval intestines. The gut bacteria were dominated by Proteobacteria. Preliminary functional analysis proved biodegradable activity on cellulose of the strains of Bacillus and xylan of the strains of Rhodococcus fascians. There were microorganisms that could degrade cellulose and xylan in the intestines of B. longissimi Gestro, and they occuped a certain dominant position. Therefore, B. longissimi can damage a variety of Palmae plants with high cellulose content, and this kind of intestinal symbiotic bacteria also provide some help for the adaptability of B. longissimi Gestro to the new environment.