马尾松抗松材线虫病无性系的抗病性评价方法研究

通过人工接种松材线虫,研究马尾松抗性候选无性系的抗病性测定评价方法。结果表明,大棚内接种能够加速病害的发生,同时在高温和干燥条件下,松树树种间反应不一样,不能真实体现出各松树种间的抗性能力,接种测定环境适合在野外。在野外接种环境下,火炬松抗性能力最强,确定无性系抗性能力对照树种应为火炬松。各松树在不同接种环境下的感病率在接种第9周后均达到最大值,在此时间内进行调查并分析植株的抗病能力较佳。抗性候选无性系间抗性差异极显著,抗性候选家系的平均健全率78.2%,抗性无性系的平均健全率76%,对照使用的马尾松健全率29.7%,火炬松健全率52.4%。结果显示马尾松抗性无性系的抗性水平高于火炬松0.45倍,为普通对照马尾松的1.63倍。

Evaluation of disease resistance of clones of Pinus massoniana to Bursaphelenchus xylophilus

The study was to evaluate the resistance of clones of Pinus massoniana to Bursaphelenchus xylophilus through inoculation of Bursaphelenchus xylophilus. The results showed that the pine wilt was seriously accelerated in the greenhouse, while the response was not the same when inoculated of Bursaphelenchus xylophilus at high temperature and dry conditions,so pine species inoculated in greenhouse did not truly reflect the disease resistance, and the best inoculation condition was in the field. The disease resistance of Pinus taeda was higher compared with other tree species, so it was chosen as the control species to identify resistance clones. The disease infection rates of all pine trees reached maximum after inoculated for 9 weeks in different inoculation conditions, so it was the best time to investigate and analyze the disease resistance of the trees. The disease resistance between candidate clones of P. massoniana was significant different. The average survival rate of candidate resistance families was 78.2%; the average survival rate of clones was 76%; the average survival rate of P. massoniana and P. taeda was 29.7% and 52.4%, respectively. The date showed that the resistance level of the clones of P. massoniana was 0.45 times higher than that of P. taeda, and 1.63 times than that of the common P. massoniana.