| ACE和ACE2在自发性高血压大鼠肾脏中的表达与作用分析 |
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| 引用本文： | 李鹏飞,苗晋锋,马畅,张伟,张源淑.ACE和ACE2在自发性高血压大鼠肾脏中的表达与作用分析[J].南京农业大学学报,2011,34(4). |
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| 作者姓名： | 李鹏飞 苗晋锋 马畅 张伟 张源淑 |
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| 作者单位： | 南京农业大学农业部动物生理生化重点开放实验室,江苏南京,210095 |
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| 基金项目： | 国家自然科学基金项目(30871838) |
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| 摘 要： | 为观察血管紧张素转化酶(ACE)和血管紧张素转化酶2(ACE2)在自发性高血压大鼠肾脏中的表达及它们与高血压形成的关系,取14周雄性Wistar-京都种大鼠(WKY)和自发性高血压大鼠(SHR)各8只,自由采食和饮水1周后测定大鼠收缩压(SBP)、舒张压(DBP)和心率(HR),称重后断头处死,取血液和肾脏组织进行如下试验:放免法测定血浆中血管紧张素Ⅱ(AngⅡ)含量,肾脏组织中ACE、AT1、ACE2和Mas mRNA表达水平的Real-time PCR分析,肾脏组织中ACE2蛋白表达的比较分析。试验结果表明:SHR大鼠SBP和DBP均显著高于WKY大鼠,而心率和体重无显著差异(P>0.05);SHR大鼠血浆中AngⅡ含量和肾脏组织中ACE mRNA表达水平均显著高于WKY大鼠(P<0.05),肾脏组织中ACE2 mRNA和蛋白表达水平均显著低于WKY大鼠(P<0.05),2组大鼠肾脏组织中AT1和Mas mRNA表达没有显著差异(P>0.05)。SHR大鼠肾脏中ACE的mRNA表达显著升高,而ACE2的mRNA和蛋白表达均显著下降。高血压时,肾脏局部组织中ACE和ACE2表达失衡,表现为ACE-AngⅡ-...
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| 关 键 词： | 自发性高血压大鼠 血管紧张素转换酶2 肾脏 高血压 |
Expression and analysis of ACE and ACE2 in the kidney of spontaneously hypertensive rats |
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| LI Peng-fei,MIAO Jin-feng,MA Chang,ZHANG Wei,ZHANG Yuan-shu.Expression and analysis of ACE and ACE2 in the kidney of spontaneously hypertensive rats[J].Journal of Nanjing Agricultural University,2011,34(4). |
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| Authors: | LI Peng-fei MIAO Jin-feng MA Chang ZHANG Wei ZHANG Yuan-shu |
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| Institution: | LI Peng-fei,MIAO Jin-feng,MA Chang,ZHANG Wei,ZHANG Yuan-shu(Key Laboratory of Animal Physiology and Biochemistry,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | To observe the expression of angiotensin-converting enzyme(ACE),ACE2 in the kidney of spontaneously hypertensive rats(SHR)and their relationship with hypertension.8 male Wistar-kyoto(WKY)rats and 8 male SHR rats which were about 300 g of weight and 14-weeks old were entrained to free access to food and tap water for one week.Systolic blood pressure(SBP),diastolic blood pressure(DBP)and heart period(HP)of all experimental rats were recorded at the end of experiment,and then all the rats were killed.The plasm... |
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| Keywords: | spontaneously hypertensive rats angiotensin-converting enzyme 2 kidney hypertension |
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