| 葡萄EST-SSR标记的开发及其应用 |
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| 引用本文： | 郭磊,上官凌飞,房经贵,刘崇怀,于华平. 葡萄EST-SSR标记的开发及其应用[J]. 南京农业大学学报, 2011, 34(4) |
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| 作者姓名： | 郭磊 上官凌飞 房经贵 刘崇怀 于华平 |
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| 作者单位： | 1. 南京农业大学园艺学院/江苏省果树品种改良与种苗繁育工程中心,江苏南京,210095
2. 中国农业科学院郑州果树研究所,河南郑州,450009 |
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| 基金项目： | 江苏省科技支撑项目(BE2010326); 第36批留学回国科研启动基金项目 |
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| 摘 要： | 从NCBI公共数据库中随机下载葡萄表达序列标签(expressed sequence tag,EST)8 925条,利用Phrap软件对这些序列进行拼接后形成5 642条序列,利用MISA软件共发掘出含有SSR位点的序列336条,共含有367个SSR位点,候选SSR位点出现的频率为6.50%。二核苷酸、三核苷酸、四核苷酸、五核苷酸和六核苷酸重复单元的SSR数目分别为79(21.53%)、83(22.62%)、29(7.9%)、60(16.35%)和116(31.61%)。利用Primer 3.0 Plus软件随机设计50对引物进行PCR扩增,用6%非变性聚丙烯酰胺凝胶电泳分析这些SSR引物的PCR扩增产物多态性。50对引物中有36对引物能在20个葡萄品种中扩增出理想的PCR产物,占总引物的72%。其中,26对引物扩增条带具有多态性。利用16对经过验证的EST-SSR引物对20个葡萄品种亲缘关系的分析获得了理想的研究结果。
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| 关 键 词： | 葡萄 EST SSR 遗传多样性 |
Development of SSR markers from grape ESTs and its application |
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| GUO Lei,SHANGGUAN Ling-fei,FANG Jing-gui,LIU Chong-huai,YU Hua-ping. Development of SSR markers from grape ESTs and its application[J]. Journal of Nanjing Agricultural University, 2011, 34(4) |
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| Authors: | GUO Lei SHANGGUAN Ling-fei FANG Jing-gui LIU Chong-huai YU Hua-ping |
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| Affiliation: | GUO Lei1,SHANGGUAN Ling-fei1,FANG Jing-gui1,LIU Chong-huai2,YU Hua-ping1(1.College of Horticulture/Fruit Grop Genetic Improvement and Seeding Propagation Engineering Center of Jiangsu Province,Nanjing Agricultural University,Nanjing 210095,China,2.Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China) |
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| Abstract: | A total of 8 925 grapevine EST(expressed sequence tag)sequences were randomly downloaded from NCBI,and were further assembled into 5 642 uniform sequences using Phrap software.These sequences were screened by using MISA software to search for SSR motifs and 367 SSR loci were identified from the 336 grape EST sequences.The frequency of EST-SSR identified was 6.50%.All the 367 SSR loci included 79(21.53%)dinucleotides,83(22.62%)trinucleotides,29(7.9%)tetranucleotides,60(16.35%)pentanucleotides,and 116(31.61%)... |
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| Keywords: | grape EST SSR genetic diversity |
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