| 狮头鹅GH基因内含子2多态性与生长和屠宰性状的相关性 |
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| 引用本文： | 陈清,赵文明,陈国宏,徐世永,宦婷婷,覃阳. 狮头鹅GH基因内含子2多态性与生长和屠宰性状的相关性[J]. 金陵科技学院学报, 2011, 27(1): 81-85 |
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| 作者姓名： | 陈清 赵文明 陈国宏 徐世永 宦婷婷 覃阳 |
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| 作者单位： | 1. 金陵科技学院动物科学与技术学院,江苏南京,210038
2. 扬州大学动物科学与技术学院,江苏扬州,225009 |
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| 摘 要： | 根据鸭生长激素基因内含子2序列设计3对引物,利用PCR-SSCP方法对狮头鹅进行了单核苷酸多态性分析。结果表明:狮头鹅GH基因内含子2内存在多态性,得到3种基因型EE、EF和FF,其中EE基因型为优势基因型,且等位基因E的频率较高。卡方检验结果表明,基因型分布符合H ardy—W e inberg平衡。基因型对6~10周龄体重、屠体重、半净膛重等11个性状有显著(P<0.05)或极显著(P<0.01)的影响,且在各个性状上,EE基因型个体的均值均高于EF和FF基因型个体。故初步推断EE基因型可以作为地方鹅种高生产性能的分子标记。
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| 关 键 词： | 狮头鹅 GH基因 PCR-SSCP 生长性状 屠宰性状 |
The Association of Polymorphisms of Intron 2 in Growth Hormone Gene with Growth and Carcass Traits in Shitou Goose |
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| CHEN Qing,ZHAO Wen-ming,CHEN Guo-hong,XU Shi-yong,HUAN Ting-ting,QIN Yang. The Association of Polymorphisms of Intron 2 in Growth Hormone Gene with Growth and Carcass Traits in Shitou Goose[J]. Journal of Jinling Institute of Technology, 2011, 27(1): 81-85 |
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| Authors: | CHEN Qing ZHAO Wen-ming CHEN Guo-hong XU Shi-yong HUAN Ting-ting QIN Yang |
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| Affiliation: | CHEN Qing1,ZHAO Wen-ming2,CHEN Guo-hong2,XU Shi-yong1,HUAN Ting-ting1,QIN Yang1(1.Jinling Institute of Technology,Nanjing 210038,China,2.Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | In this study the primers for intron 2 in GH gene are designed according to the homologous sequence of duck,and single nucleotide polymorphism(SNP) of growth hormone(GH) gene in Shitou goose is detected by PCR-SSCP method.Three genotypes(EE,EF and FF) are found in Shitou goose,EE genotype is dominated in population,and the frequency of allele E is higher.The results of Chi Square test show that the frequencies of GH gene fit with Hardy-Weinberg equilibrium in Shitou goose.The gene is significantly correlate... |
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| Keywords: | Shitou goose GH gene PCR-SSCP growth traits carcass traits |
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