| 产表面活性剂石油降解菌的筛选及发酵条件优化 |
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| 引用本文： | 张娜,郑青松,刘玲,刘兆普,牛丹丹,姚瑶,王博.产表面活性剂石油降解菌的筛选及发酵条件优化[J].南京农业大学学报,2011,34(4). |
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| 作者姓名： | 张娜 郑青松 刘玲 刘兆普 牛丹丹 姚瑶 王博 |
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| 作者单位： | 南京农业大学资源与环境科学学院/江苏省海洋生物学重点实验室,江苏南京,210095 |
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| 基金项目： | 国家公益性行业(农业)科研专项(200903001-05) |
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| 摘 要： | 从胜利油田受石油污染的土壤中筛选出能产生表面活性剂的石油降解菌,对其进行初步鉴定,并探讨了其降解原油的性能与生长条件。经过富集培养、平板筛选、血平板筛选、排油圈测定,成功分离筛选出1株产生表面活性剂的石油降解菌株X-1。经形态观察、生理生化试验和16S rDNA序列分析等鉴定其为芽孢杆菌属(Bacillus sp.),培养6 d时原油降解率为30.04%。通过硅胶板薄层层析初步判定表面活性剂粗品中含有脂肽、脂蛋白类物质。菌株生长的最适温度为32℃,最适pH为7.0,最适盐度为2 g.L-1 NaCl,最佳碳源为淀粉,最佳氮源为蛋白胨。
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| 关 键 词： | 石油降解菌 降解特性 生物表面活性剂 筛选 优化 |
Isolation of oil-degrading bacteria with biosurfactant production and its fermentation condition optimization |
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| ZHANG Na,ZHENG Qing-song,LIU Ling,LIU Zhao-pu,NIU Dan-dan,YAO Yao,WANG Bo.Isolation of oil-degrading bacteria with biosurfactant production and its fermentation condition optimization[J].Journal of Nanjing Agricultural University,2011,34(4). |
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| Authors: | ZHANG Na ZHENG Qing-song LIU Ling LIU Zhao-pu NIU Dan-dan YAO Yao WANG Bo |
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| Institution: | ZHANG Na,ZHENG Qing-song,LIU Ling,LIU Zhao-pu,NIU Dan-dan,YAO Yao,WANG Bo(College of Resources and Environmental Sciences/Key Laboratory of Marine Biology of Jiangsu Province,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | An oil-degrading bacteria with biosurfactant production was isolated from petroleum-contaminated soil of Shengli Oil Field in Shandong Province,and preliminary identification was conducted on it.We also studied its degradation characteristics and growth conditions.By enrichment cultivation,plate screening,blood plate screening and determination of oil discharge circle,we successfully isolated strain X-1.According to its morphological,physiological,biochemical features and 16S rDNA sequence analysis,strain X... |
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| Keywords: | oil-degrading bacterium degradation characteristics biosurfactant screening optimization |
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