| 冷胁迫下不结球白菜BcSLAC1基因克隆和表达分析及ABA和H_2O_2在调控气孔运动中的作用 |
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| 引用本文： | 陈海旺,侯喜林,李英,王枫,杨学东,张硕.冷胁迫下不结球白菜BcSLAC1基因克隆和表达分析及ABA和H_2O_2在调控气孔运动中的作用[J].南京农业大学学报,2011(4). |
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| 作者姓名： | 陈海旺 侯喜林 李英 王枫 杨学东 张硕 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室/农业部南方蔬菜遗传改良重点开放实验室; |
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| 基金项目： | 江苏省自然科学基金项目(BK2009311); 江苏省农业科技自主创新资金项目(CX(09)604) |
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| 摘 要： | 采用RT-PCR方法首次获得不结球白菜BcSLAC1基因,该基因核甘酸序列全长1 668 bp,其开放阅读框编码555个氨基酸。与已公布的拟南芥SLAC1基因有较高的同源性。系统进化树分析表明:与SLAHs基因家族其他基因相比,BcSLAC1基因与拟南芥SLAC1基因有更近的遗传距离。在冷胁迫条件下利用气孔开张度测量和荧光显微技术分析表明:冷胁迫下H2O2可能作为下游信号分子参与了保卫细胞中ABA信号转导。实时定量RT-PCR检测表明,冷胁迫下BcSLAC1基因表达量升高,同时用ABA处理后,BcSLAC1基因表达量比抗坏血酸处理后明显上升,说明ABA对BcSLAC1基因的诱导作用和ABA诱导产生的H2O2有关。
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| 关 键 词： | 不结球白菜 BcSLAC1基因 气孔运动 荧光观察 实时定量PCR |
Cloning,expression of BcSLAC1 induced by cold stress in Brassica campestris ssp.chinensis and the role in regulating stomatal movements of ABA and H_2O_2 |
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| CHEN Hai-wang,HOU Xi-lin,LI Ying,WANG Feng,YANG Xue-dong,ZHANG Shuo.Cloning,expression of BcSLAC1 induced by cold stress in Brassica campestris ssp.chinensis and the role in regulating stomatal movements of ABA and H_2O_2[J].Journal of Nanjing Agricultural University,2011(4). |
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| Authors: | CHEN Hai-wang HOU Xi-lin LI Ying WANG Feng YANG Xue-dong ZHANG Shuo |
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| Institution: | CHEN Hai-wang,HOU Xi-lin,LI Ying,WANG Feng,YANG Xue-dong,ZHANG Shuo(State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Southern Vegetable Crop Genetics Improvement,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | BcSLAC1 gene was firstly amplified from non-heading Chinese cabbage by RT-PCR method.The nucleotide sequence length of its open reading frame(ORF)was 1 668 bp and encoded 555 amino acid residues,and it showed high homology to Arabidopsis thaliana SLAC1 gene.Phylogenetic tree analysis demonstrated that BcSLAC1 gene had a more recent genetic distance with Arabidopsis thaliana SLAC1 gene compared to the others of SLAHs family.In the cold stress condition,stomatcal aperture bioassay and fluorescence microscopy ... |
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| Keywords: | Brassica campestris ssp chinensis BcSLAC1 gene stomatal movement fluorescence observation real-time quantitative PCR |
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