| 公牛HSP基因多态性与其精液品质及后裔性能的相关分析 |
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| 引用本文： | 廖旭东,崔群维,周璇,王振云,郭宁,刘延鑫,王雅春,王根林.公牛HSP基因多态性与其精液品质及后裔性能的相关分析[J].南京农业大学学报,2011,34(4). |
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| 作者姓名： | 廖旭东 崔群维 周璇 王振云 郭宁 刘延鑫 王雅春 王根林 |
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| 作者单位： | 1. 南京农业大学动物科技学院,江苏南京,210095
2. 中国农业大学动物科技学院,北京,100193 |
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| 基金项目： | 国家科技支撑计划项目(2006BAD04A01,2006BAD04A12); 江苏省科技支撑计划项目(BE2008306-2) |
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| 摘 要： | 以142头公牛为研究对象,以HSP为耐热性状的候选基因,分别扩增125和233 bp 2个片段,采用PCR-SSCP方法检测HSP基因多态性,并对PCR产物测序和序列分析。结果表明:HSPA8存在T3358C转换突变,该突变使缬氨酸(Val)变为异亮氨酸(Ile),产生2种基因型AA和AB,频率分别为0.577 5和0.422 5;HSPA1A发现A6201C和T6202A颠换突变,其中T6202A突变使苯丙氨酸(Phe)变为异亮氨酸(Ile),产生了5种基因型AA、AB、BB、AC和BC,频率分别为0.345 1、0.493 0、0.084 5、0.035 2和0.042 2。应用SPSS17.0软件采用最小二乘拟合线性模型,分析基因座位不同标记基因型与后裔测定性能和精液品质的相关性,结果表明:1)在HSPA8,AA基因型个体产量育种值显著高于AB型(P<0.05);在HSPA1A,BB基因型个体产量育种值显著高于AC型(P<0.05),AB基因型个体蛋白育种值显著高于AC型(P<0.05)。2)在HSPA8,精子密度、精子活力和顶体完整率AA均显著高于AB(P<0.05),而精子畸形率AA显著低于AB(P...
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| 关 键 词： | 公牛 热应激 精液品质 后裔性能 热休克蛋白基因(HSP) |
The correlation analysis between bulls HSP gene polymorphism and semen quality and progeny performance |
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| LIAO Xu-dong,CUI Qun-wei,ZHOU Xuan,WANG Zhen-yun,GUO Ning,LIU Yan-xin,WANG Ya-chun,WANG Gen-lin.The correlation analysis between bulls HSP gene polymorphism and semen quality and progeny performance[J].Journal of Nanjing Agricultural University,2011,34(4). |
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| Authors: | LIAO Xu-dong CUI Qun-wei ZHOU Xuan WANG Zhen-yun GUO Ning LIU Yan-xin WANG Ya-chun WANG Gen-lin |
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| Institution: | LIAO Xu-dong1,CUI Qun-wei1,ZHOU Xuan1,WANG Zhen-yun1,GUO Ning1,LIU Yan-xin1,WANG Ya-chun2,WANG Gen-lin1(1.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China,2.College of Animal Science and Technology,China Agricultural University,Beijing 100193,China) |
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| Abstract: | In this experiment,125 bp and 233 bp fragments of heat shock protein gene(HSP)named HSPA8 and HSPA1A from 142 bulls were amplified by polymerase chain reaction(PCR),respectively.The genetic polymorphisms in the population were detected by single-strand conformational polymorphism(SSCP),and the PCR products were sequenced and sequence analysis was performed.The results indicated that T3358C transition mutations in HSPA8,and A6201C and T6202A transvertion mutations in HSPA1A were founded.T3358C mutation resul... |
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| Keywords: | bulls heat stress semen quality progeny performance heat shock protein gene(HSP) |
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