| 11种植物提取物抗烟草花叶病毒活性研究 |
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| 引用本文： | 祁进康, 邹正彪, 王德艳, 毕再涛, 钱瑞, 陈永对, 闫晓慧. 11种植物提取物抗烟草花叶病毒活性研究[J]. 西南林业大学学报, 2017, 37(6): 129-134.doi:10.11929/j.issn.2095-1914.2017.06.021 |
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| 作者姓名： | 祁进康 邹正彪 王德艳 毕再涛 钱瑞 陈永对 闫晓慧 |
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| 作者单位： | 1. 西南林业大学生物多样性保护与利用学院, 云南省森林灾害预警与控制重点实验室，云南 昆明 650224;2. 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室，云南 昆明 650223 |
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| 基金项目： |
摘要:为开发环境友好型的植物源抗病毒剂，以烟草花叶病毒(TMV)为供试病毒，采用半叶枯斑法，研究了11种植物不同溶剂提取物的抗TMV活性。结果表明：当浓度为1 mg/mL时，臭灵丹石油醚提取物、红花月见草乙酸乙酯提取物、臭灵丹水层提取物对TMV增殖有显著抑制(P＜0.05)活性抑制率分别为68.47%、80.19%、94.57%；臭灵丹石油醚提取物、钻叶紫菀乙酸乙酯提取物、臭灵丹水提取物对TMV有较强的钝化活性，抑制率分别为80.00%、76.38%、83.33%。TAS-ELISA检测表明，臭灵丹、风轮草、珊瑚樱、钻叶紫菀、益母草、拔毒散等植物提取物对普通烟K326内病毒增殖具有显著抑制作用(P＜0.05)，并且明显好于阳性对照药剂宁南霉素。 |
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| 摘 要： | 为开发环境友好型的植物源抗病毒剂，以烟草花叶病毒(TMV)为供试病毒，采用半叶枯斑法，研究了11种植物不同溶剂提取物的抗TMV活性。结果表明：当浓度为1 mg/mL时，臭灵丹石油醚提取物、红花月见草乙酸乙酯提取物、臭灵丹水层提取物对TMV增殖有显著抑制(P＜0.05)活性抑制率分别为68.47%、80.19%、94.57%；臭灵丹石油醚提取物、钻叶紫菀乙酸乙酯提取物、臭灵丹水提取物对TMV有较强的钝化活性，抑制率分别为80.00%、76.38%、83.33%。TAS-ELISA检测表明，臭灵丹、风轮草、珊瑚樱、钻叶紫菀、益母草、拔毒散等植物提取物对普通烟K326内病毒增殖具有显著抑制作用(P＜0.05)，并且明显好于阳性对照药剂宁南霉素。
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| 关 键 词： | 烟草花叶病毒 抗TMV活性 半叶枯斑法 叶圆片法 检测 |
| 收稿时间： | 2017-03-30 |
Anti-Tobacco Mosaic Virus Activity of 11 Plant Extracts |
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| Jinkang Qi, Zhengbiao Zou, Deyan Wang, Zaitao Bi, Rui Qian, Yongdui Chen and Xiaohui Yan. Anti-Tobacco Mosaic Virus Activity of 11 Plant Extracts[J]. Journal of Southwest Forestry University, 2017, 37(6): 129-134.doi:10.11929/j.issn.2095-1914.2017.06.021 |
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| Authors: | Jinkang Qi Zhengbiao Zou Deyan Wang Zaitao Bi Rui Qian Yongdui Chen Xiaohui Yan |
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| Affiliation: | 1. Key laboratory of Forest Disaster Warning and Control of Yunnan, College of Biodiversity Conservation and Utilization, Southwest Forestry University, Kunming Yunnan 650224, China;2. Key Laboratory of Agricultural Biotechnology of Yunnan, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming Yunnan 650223, China |
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| Abstract: | In order to develop environmentally friendly antiviral agents from plants, the anti tobacco mosaic virus(TMV) activities of extracts from 11 plants were studied by half-leaf method. The results showed that petroleum ether extracts from Laggera pterodonta, the ethyl acetate extracts from Oenothera rosea and the water extracts from L.pterodonta showed potent antiviral proliferation activities with inhibition rates of 68.47%, 80.19% and 94.57% respectively at the concentration of 1 mg/mL. The petroleum ether extracts from L.pterodonta, ethyl acetate extracts from Aster subulatus and water extracts from L.pterodonta showed prominent inactivation activities against TMV with inhibition rate 80.00%, 76.38% and 83.33%, respectively. According to TAS-ELISA experiment, extracts from L.pterodonta, Clinopodium polycephalum, Solanum pseudo-capsicum, A.subulatus, Leonurus Artemisia, Sida szechuensis showed significant anti-TMV proliferation activities in Nicotiana tabacum CV. K326. The antivirus components from these plants were worth further investigation. |
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| Keywords: | tobacco mosaic virus (TMV) anti-TMV activity half-leaf method TAS-ELISA detection |
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