Molecular Taxonomy of Conogethes punctiferalis and Conogethes pinicolalis(Lepidoptera: Crambidae) Based on Mitochondrial DNA Sequences |
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作者姓名: | WANG Jing, ZHANG Tian-tao, WANG Zhen-ying, HE Kang-lai, LIU Yong, LI Jing |
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作者单位: | [1]State Key Laboratory for Biology of the Plant Diseases and lnsect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China; [2]College of Plant Protection, Shandong Agricultural University, Tai 'an 271018, P.R. China |
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基金项目: | supported by China Agriculture Research System(CARS-02); Beijing Municipal Sci-Tech Program(Z111100056811009) |
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摘 要: | Conogethes punctiferalis(Guenée)(Lepidoptera: Crambidae) was originally considered as one species with fruit-feeding type(FFT) and pinaceae-feeding type(PFT), but it has subsequently been divided into two different species of Conogethes punctiferalis and Conogethes pinicolalis. The relationship between the two species was investigated by phylogenetic reconstruction using maximum-likelihood(ML) parameter estimations. The phylogenetic tree and network were constructed based upon sequence data from concatenation of three genes of mitochondrial cytochrome c oxidase subunits I, II and cytochrome b which were derived from 118 samples of C. punctiferalis and 24 samples of C. pinicolalis. The phylogenetic tree and network showed that conspecific sequences were clustering together despite intraspecific variability. Here we report the results of a combined analysis of mitochondrial DNA sequences from three genes and morphological data representing powerful evidence that C. pinicolalisand C. punctiferalis are significantly different.
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关 键 词: | DNA序列 桃蛀螟 线粒体 鳞翅目 草螟科 分子分类学 细胞色素C氧化酶 系统发育树 |
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