高盐低pH值法提取玉米基因组DNA的研究

优化建立了基于玉米叶片的高盐低pH值法提取玉米基因组DNA,经琼脂糖电泳检测表明，提取的DNA主带清晰、无降解现象、质量好，满足基于SSR标记的5色荧光电泳检测系统。此方法比目前提取植物基因组DNA常用的CTAB法和SDS法具有成本低、步骤简单、不使用酚、氯仿、异戊醇等有毒有机试剂的优点，是提取玉米基因组DNA的一种较好的方法。

Studies on Genomic DNA Extraction of Maize with High Salt, Low pH Method

37 waxy corn landraces in Yunnan and 5 main heterotic groups of maize inbreds in domestic hybrid maize production were used to analyze their genetic diversities and relations using SSR markers and divided group. The results showed that 20 pairs core primers out of 63 pairs of core SSR primers which were used by other labs were selected which gave stable and high PIC value. 225 alleles were amplified in the 37 waxy corn landraces by the core primers, average polymorphism information content(PIC) was 0.87, average marker index(MI) was 9.91, Shannon's information index(I) was 0.54. Genetic similarity and cluster analysis showed that 37 waxy corn landraces were divided into 5 groups and 13 sub-groups.Genetic distance was close between a few of waxy corn landraces and 5 main heterotic groups was far between most waxy corn landraces and 5 main heterotic grouping, most waxy corn landraces were divided into many single groups. Genetic base was very wide within waxy corn landraces of Yunnan. The genetic similarity was significantly different between different waxy corn landraces from same or different districts and 5 main heterotic groups.